耐盐性需要协调的反应,包括根中的盐排除和叶片中的盐的组织/细胞分隔。我们研究了两种不同基因型绿豆的根中盐离子运输和组织对Na和Cl-的耐受性的可能控制点,耐盐JadeAU和盐敏感性BARIMung-6,生长在非盐水和盐水(75mMNaCl)土壤中。Cryo-SEMX射线微区分析用于确定Na的浓度,Cl,K,Ca,Mg,P,和S在根中的各种细胞类型中,与间质障碍的发展有关,以及与光合性能相关的叶片。JadeAU通过在内部皮质中积累更高的[Na]表现出优异的盐排斥,内皮,与BARIMung-6相比,木质部血管中的[Na]减少,皮质细胞液泡中的[Cl]积累。玉AU在根细胞中保持比BARIMung-6更高的[K]。在树叶中,与BARIMung-6相比,JadeAU在叶绿体中保持较低的[Na]和[Cl],并优先在叶肉细胞中积累[K],从而获得更高的光合效率。玉AU的耐盐性与芽Na和Cl排除有关,有效调节叶绿体中Na和Cl的积累,和维持根和叶叶肉细胞中的高钾。
Salinity tolerance requires coordinated responses encompassing salt exclusion in roots and tissue/cellular compartmentation of salt in leaves. We investigated the possible control points for salt ions transport in roots and tissue tolerance to Na+ and Cl- in leaves of two contrasting mungbean genotypes, salt-tolerant Jade AU and salt-sensitive BARI Mung-6, grown in nonsaline and saline (75 mM NaCl) soil. Cryo-SEM X-ray microanalysis was used to determine concentrations of Na, Cl, K, Ca, Mg, P, and S in various cell types in roots related to the development of apoplastic barriers, and in leaves related to photosynthetic performance. Jade AU exhibited superior salt exclusion by accumulating higher [Na] in the inner cortex, endodermis, and pericycle with reduced [Na] in xylem vessels and accumulating [Cl] in cortical cell vacuoles compared to BARI Mung-6. Jade AU maintained higher [K] in root cells than BARI Mung-6. In leaves, Jade AU maintained lower [Na] and [Cl] in chloroplasts and preferentially accumulated [K] in mesophyll cells than BARI Mung-6, resulting in higher photosynthetic efficiency. Salinity tolerance in Jade AU was associated with shoot Na and Cl exclusion, effective regulation of Na and Cl accumulation in chloroplasts, and maintenance of high K in root and leaf mesophyll cells.