■本研究旨在研究溶解在二甲基亚砜(DMSO)中的薄荷醇对雄性大鼠腹膜内(IP)注射戊四氮(PTZ)引起的实验性癫痫样活性的保护作用。
■将30只体重为200-250g的成年雄性Wistar大鼠随机分为5组。对照动物接受生理盐水(200μL),其余四个组群被认为是治疗。薄荷醇溶解在DMSO中,并以100、200和400mg/kg的剂量腹膜内注射入第一个,第二,和第三组(M100,M200和M400V=200μL),分别。第四处理用溶剂(200μL)注入。动物被麻醉,然后进行了颅骨手术,并在海马碳酸酐酶1(CA1)区域的放射层中植入了记录电极(AP=-2.76mm,ML=-1.4mm和DV=3mm)。癫痫发作活性由PTZ(IP)诱导,并通过使用eTrace程序计数和测量尖峰的幅度10分钟来评估。
■观察到薄荷醇显着降低PTZ诱导的癫痫样活动的活动水平,并以剂量依赖性方式对DMSO的促惊厥作用发挥保护和抑制作用。
■薄荷醇可用作预防癫痫发作的佐剂。
■二甲基亚砜(DMSO)诱导前惊厥作用,显著增加尖峰计数。薄荷醇100毫克/千克也刺激癫痫发作活动,导致峰值数量大幅增加。薄荷醇200和400毫克/千克表现出抑制作用,减少癫痫发作活动和尖峰计数。
■在这项研究中,我们探索了薄荷醇的潜在保护作用,溶解在二甲基亚砜(DMSO)中,实验诱导雄性大鼠癫痫样活动。我们的研究涉及30只成年雄性Wistar大鼠,分为五组。对照组给予生理盐水,其余四组用不同剂量的薄荷醇在DMSO中治疗。老鼠接受了手术,并将电极植入海马区进行记录。使用戊四氮(PTZ)诱导癫痫发作活动,我们观察到薄荷醇,以不同的剂量给药,显著降低PTZ引发的癫痫样活动水平。值得注意的是,薄荷醇还对DMSO的前惊厥作用具有保护和抑制作用,这种效应是剂量依赖性的。简单地说,我们的研究结果表明,薄荷醇有可能作为预防癫痫发作的额外治疗方法.这意味着加入薄荷醇,特别是在特定剂量下,可能对癫痫事件有保护性影响。这项研究为潜在的治疗干预提供了一个有希望的途径,强调进一步探索薄荷醇在癫痫预防中的作用的重要性。最终,我们的研究为在制定减轻癫痫发作影响的策略时考虑薄荷醇的重要组成部分打开了大门.
UNASSIGNED: This research aims to investigate the protective action of
menthol dissolved in dimethyl sulfoxide (DMSO) on experimental epileptiform activity induced by the intraperitoneal (IP) injection of pentylenetetrazol (PTZ) in male rats.
UNASSIGNED: Thirty adult male Wistar rats weighing 200-250 g were randomly assigned to five equal groups. The control animals received normal saline (200 μL) and the rest four cohorts were considered as treatment.
Menthol was dissolved in DMSO and intraperitoneally injected at the doses of 100, 200, and 400 mg/kg into the first, second, and third groups (M100, M200, and M400 V=200 μL), respectively. The fourth treatment was injected with the solvent (200 μL). The animals were anesthetized, then underwent cranial surgery and a recording electrode was implanted in the stratum radiatum of the hippocampal carbonic anhydrase 1 (CA1) region (AP=-2.76 mm, ML=-1.4 mm and DV=3 mm). The seizure activity was induced by PTZ (IP) and assessed by counting and measuring amplitudes of the spikes for 10 minutes using the eTrace program.
UNASSIGNED: Menthol was observed to significantly reduce the activity level of PTZ-induced epileptiform activity, as well as exert a protective and inhibitory action on proconvulsant effect of DMSO in a dose-dependent manner.
UNASSIGNED: Menthol can potentially be used as an adjuvant to prevent seizure activity.
UNASSIGNED: Dimethyl sulfoxide (DMSO) induces proconvulsant effects, significantly increasing spike counts.Menthol 100 mg/kg also stimulates seizure activity, leading to a substantial spike count increase.Menthol 200 and 400 mg/kg exhibit inhibitory effects, decreasing seizure activity and spike counts.
UNASSIGNED: In this study, we explored the potential protective effects of menthol, dissolved in dimethyl sulfoxide (DMSO), on experimentally induced epileptiform activity in male rats. Our research involved thirty adult male Wistar rats, divided into five groups. While the control group received normal saline, the remaining four groups were treated with different doses of menthol in DMSO. The rats underwent surgery, and electrodes were implanted in the hippocampal region for recording. Using pentylenetetrazol (PTZ) to induce seizure activity, we observed that menthol, administered at varying doses, significantly reduced the level of epileptiform activity triggered by PTZ. Notably,
menthol also demonstrated a protective and inhibitory effect on the proconvulsant action of DMSO, and this effect was dose-dependent. In simpler terms, our findings suggest that menthol has the potential to be used as an additional treatment to prevent seizure activity. This means that incorporating menthol, especially at specific doses, may offer a protective influence against epileptic events. This research sheds light on a promising avenue for potential therapeutic interventions, emphasizing the importance of further exploration of menthol\'s role in epilepsy prevention. Ultimately, our study opens the door to considering menthol as a valuable component in the development of strategies to mitigate the impact of seizures.