微生物操纵策略变得不间断,生物膜在扩大毒力以引起持久感染方面发挥了完美的作用。本研究旨在评估使用从Menthalongifolia叶提取物(M盘E)产生的功能化银纳米颗粒的生态友好配方,用于治疗生物膜产生的微生物。在不同强度的AgNO3(1mM,2mM,3mM,和4mM)。用分光光度法(450nm)观察到长叶分枝杆菌银纳米颗粒(M+AgNP)的合成,表明M+AgNP(4mM)具有最高的吸光度。各种技术,例如,傅里叶变换红外光谱(FTIR),动态光散射(DLS),zeta电位(ZP),X射线衍射(XRD)扫描电子显微镜(SEM),和透射电子显微镜(TEM)用于表征MAgNPs。在本研究中,Kirby-Bauer方法显示4mM是最有害的浓度。MAgNPs的MIC和MBC值为0.62μg/mL和1.25μg/mL,0.03μg/mL和0.078μg/mL,和0.07μg/mL和0.15μg/mL针对先前分离和鉴定的大肠杆菌临床菌株,铜绿假单胞菌,肺炎克雷伯菌,和金黄色葡萄球菌,分别。此外,通过组织培养板(TCP)检测MAgNP抗生物膜活性,显示生物膜抑制高达87.09%,85.6%,83.11%,和75.09%的大肠杆菌,铜绿假单胞菌,K.肺炎,和金黄色葡萄球菌,分别。草药合成的银纳米颗粒(MAgNPs)倾向于具有优异的抗菌和抗生物膜性能,并且有望用于其他生物医学应用,包括消除一些生物膜。为了我们最好的知识,这是关于使用绿色合成的银纳米颗粒MAgNP作为抗生物膜剂的第一个研究,表明这种材料含有抗生素,治疗性的,和工业应用。
Microbes maneuver strategies to become incessant and biofilms perfectly play a role in scaling up virulence to cause long-lasting infections. The present study was designed to assess the use of an eco-friendly formulation of functionalized silver nanoparticles generated from
Mentha longifolia leaf extract (MℓE) for the treatment of biofilm-producing microbes. Nanoparticles synthesized using MℓE as a reducing agent were optimized at different strengths of AgNO3 (1 mM, 2 mM, 3 mM, and 4 mM). Synthesis of M. longifolia silver nanoparticles (MℓAgNPs) was observed spectrophotometrically (450 nm) showing that MℓAgNPs (4 mM) had the highest absorbance. Various techniques e.g., Fourier transforms Infrared spectroscopy (FTIR), Dynamic light scattering (DLS), zeta potential (ZP), X-ray Diffraction (XRD), scanning electron microscope (SEM), and transmission electron microscope (TEM) were used to characterize MℓAgNPs. In the present study, the Kirby-Bauer method revealed 4mM was the most detrimental conc. of MℓAgNPs with MIC and MBC values of 0.62 μg/mL and 1.25 μg/mL, 0.03 μg/mL and 0.078 μg/mL, and 0.07 μg/mL and 0.15 μg/mL against previously isolated and identified clinical strains of Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus aureus, respectively. Moreover, the MℓAgNP antibiofilm activity was examined via tissue culture plate (TCP) assay that revealed biofilm inhibition of up to 87.09%, 85.6%, 83.11%, and 75.09% against E. coli, P. aeruginosa, K. pneumonia, and S. aureus, respectively. Herbal synthesized silver nanoparticles (MℓAgNPs) tend to have excellent antibacterial and antibiofilm properties and are promising for other biomedical applications involving the extrication of irksome biofilms. For our best knowledge, it is the first study on the use of the green-synthesized silver nanoparticle MℓAgNP as an antibiofilm agent, suggesting that this material has antibiotic, therapeutic, and industrial applications.