猪链球菌是世界范围内流行的猪的主要病原体之一,疫苗的开发将有助于有效控制猪链球菌病。在这项研究中,我们评估了三种膜相关蛋白的潜力,组氨酸激酶(HK),糖基转移酶家族2(Gtf-2)和磷酸结合蛋白(PsbP)作为亚单位疫苗。生物信息学分析表明,猪链球菌中的ABC蛋白高度保守。为了验证这些蛋白质在动物模型中的保护作用,重组蛋白HK,Gtf-2和PsbP分别用于免疫BALB/c小鼠。结果表明,这些蛋白在小鼠体内免疫可以有效诱导强体液免疫应答,保护小鼠免受猪链球菌感染引起的细胞因子风暴,对致死剂量的猪链球菌感染有显著的保护作用。此外,具有调理活性的抗体存在于重组蛋白抗血清中,以帮助吞噬细胞杀死猪链球菌。总的来说,这些结果表明,这些重组蛋白对猪链球菌感染均具有良好的免疫保护作用,可以代表有希望的候选抗原用于猪链球菌亚单位疫苗。
Streptococcus suis is one of the major pathogens of pigs circulating worldwide, and the development of vaccines will help to effectively control streptococcosis in swine. In this study, we evaluated the potential of three membrane associated proteins, histidine kinase (HK), glycosyltransferase family 2 (Gtf-2) and phosphate binding protein (PsbP) of S. suis as subunit vaccines. Bioinformatics analysis shows that protein ABC is highly conserved in S. suis. To verify the protective effects of these proteins in animal models, recombinant protein HK, Gtf-2 and PsbP were used to immunize BALB/c mice separately. The results showed that these proteins immunization in mice can effectively induce strong humoral immune responses, protect mice from cytokine storms caused by S. suis infection, and have a significant protective effect against lethal doses of S. suis infection. Furthermore, antibodies with opsonic activity exist in the recombinant proteins antiserum to assist phagocytic cells in killing S. suis. Overall, these results indicated that these recombinant proteins all elicit good immune protective effect against S. suis infection and can be represent promising candidate antigens for subunit vaccines against S. suis.