水稻(OryzasativaL.)是世界上种植最广泛的作物之一。是全球一半以上人口的主食。根结线虫(RKN),根结线虫属。,尤其是M.Graminicola,似乎是重要的水稻害虫,这使它们成为该作物中经济上最重要的植物寄生线虫。RKN通过使宿主细胞分化成肥大而在gall中发展出摄食位点,多核,被称为巨细胞的代谢活跃细胞。这种放牧框架为线虫提供了恒定的食物来源,允许它发育成一个肥沃的雌性,并在宿主根内完成其生命周期。M.graminicola效应蛋白参与线虫寄生,包括先锋基因,在早期的研究中具有功能特征。对Meloidogynegraminicola蛋白靶标进行了分子建模和对接研究,如β-1,4-内切葡聚糖酶,果胶酸裂解酶,磷脂酶B样蛋白,和G蛋白偶联受体激酶,为了了解β-D-半乳糖醛酸的结合亲和力,2,6,10,15,19,23-六甲基四烷,(2S)-2-氨基-3-苯基丙酸,和4-O-β-D-吡喃半乳糖基-α-D-吡喃葡萄糖抗水稻配体分子。这项研究发现了植物-线虫相互作用的重要分子方面以及受M.graminicola感染的水稻植物调节的候选效应蛋白。据我们所知,这是第一个描述M.graminicola对寄主寄生的分子适应的研究。
Rice (Oryza sativa L.) is one of the most widely grown crops in the world, and is a staple food for more than half of the global total population. Root-knot nematodes (RKNs), Meloidogyne spp., and especially M. graminicola, seem to be significant rice pests, which makes them the most economically important plant-parasitic nematode in this crop. RKNs develop a feeding site in galls by causing host cells to differentiate into hypertrophied, multinucleate, metabolically active cells known as giant cells. This grazing framework gives the nematode a constant food source, permitting it to develop into a fecund female and complete its life cycle inside the host root. M. graminicola effector proteins involved in nematode parasitism, including pioneer genes, were functionally characterized in earlier studies. Molecular modelling and docking studies were performed on Meloidogyne graminicola protein targets, such as β-1,4-endoglucanase, pectate lyase, phospholipase B-like protein, and G protein-coupled receptor kinase, to understand the binding affinity of Beta-D-Galacturonic Acid, 2,6,10,15,19,23-hexamethyltetracosane, (2S)-2-amino-3-phenylpropanoic acid, and 4-O-Beta-D-Galactopyranosyl-Alpha-D-Glucopyranose against ligand molecules of rice. This study discovered important molecular aspects of plant-nematode interaction and candidate effector proteins that were regulated by M. graminicola-infected rice plants. To the best of our knowledge, this is the first study to describe M. graminicola\'s molecular adaptation to host parasitism.