在实验室实验中很少评估微塑料和吸附的多环芳烃在社区水平上的影响,尽管与传统上单一物种集中的毒理学实验相比,它们在更好地反映自然条件方面具有明显的优势。在目前的研究中,聚氯乙烯和chrysene的多方面影响,单独或联合行动,关于一般海洋底栖动物,但是特别关注自由生活的海洋线虫群落在微观世界进行的实验室实验中进行了测试。将底栖动物暴露于两种聚氯乙烯(5和10mg。kg-1干重\'DW\')和chrysene(37.5和75ng。g-1DW)浓度,分别,以及两种化合物的混合物,30天。结果突出显示了所有中胚底类群的丰度显着下降,包括线虫,当单独添加时,这些化合物的剂量直接增加。添加chrysene粘附到微塑料上,使沉积物基质更胶粘,因此,在一般的甲胎动物群体中引起更高的死亡率。此外,在单独添加两种化合物后,线虫经历了强烈的重组阶段,导致敏感线虫的消失,并被耐受性分类群取代。然而,对照与聚氯乙烯和菊花混合物之间线虫组成的相似性表明,后者的毒性可以通过与前一种污染物的物理结合而减弱。线虫群落中功能性状的其他变化是雌性的生育力下降和优势种暴露于两种污染物后的咽部泵送能力增加。后者的结果也得到了其他毒物动力学分析和计算机模拟的支持。
The effects of microplastics and sorbed polycyclic aromatic hydrocarbons at community levels were rarely assessed in laboratory experiments, despite their obvious advantage in reflecting better the natural conditions compared to traditionally single species-focused toxicological experiments. In the current study, the multifaceted effects of polyvinyl chloride and chrysene, acting alone or combined, on general marine meiobenthos, but with a special focus on free-living marine nematode communities were tested in a laboratory experiment carried in microcosms. The meiobenthos was exposed to two polyvinyl chloride (5 and 10 mg.kg-1 Dry Weight \'DW\') and chrysene (37.5 and 75 ng.g-1 DW) concentrations, respectively, as well as to a mixture of both compounds, for 30 days. The results highlighted a significant decrease in the abundance of all meiobenthic generic groups, including nematodes, directly with increasing dosages of these compounds when added alone. The addition of chrysene adheres to microplastics, making the sediment matrix glueyer, hence inducing greater mortality among generic meiobenthic groups. Moreover, the nematofauna went through a strong restructuring phase following the exposure to both compounds when added alone, leading to the disappearance of sensitive nematodes and their replacement with tolerant taxa. However, the similarity in nematofauna composition between control and polyvinyl chloride and chrysene mixtures suggests that the toxicity of the latter could be attenuated by its physical bonding to the former pollutant. Other changes in the functional traits within the nematode communities were a decline in the fertility of females and an increase of the pharyngeal pumping power following exposure to both pollutants for the dominant species. The latter results were also supported by additional toxicokinetics analyses and in silico modeling.