■对低收入国家新兴的严重急性呼吸道综合症冠状病毒2(SARS-CoV-2)变种实施基于基因组的监测,它们的分子和测序能力不足,疫苗储存有限,对公共卫生构成挑战。迄今为止,在SARS-CoV-2变异可能出现的地区进行分子研究的证据很少.我们报告了针对移民的实验性SARS-CoV-2分子监测计划的结果,难民,寻求庇护者通过地中海通过意大利到达欧洲。
我们对2021年2月至2022年5月在西西里岛入境点收集的移民数据进行了描述性分析。这些入口点与完全具备分子分析能力的实验室网络相结合,该公司进行了下一代测序,并使用Nextclade和2019年穿山甲冠状病毒病(COVID-19)工具进行进化枝/谱系分配。
■我们获得了472个全长SARS-CoV-2序列,并鉴定了属于31个不同谱系的12个独特进化枝。δ变异占所有基因组的43.6%,其次是进化枝21D(Eta)和20A(25.4%和11.4%,分别)。值得注意的是,一些已确定的血统(A.23.1,A.27和A.29)预测将其引入迁徙地区。突变分析使我们能够鉴定出617个不同的氨基酸取代,156个氨基酸缺失,7个停止密码子,和6个氨基酸插入。最后,我们强调了移民原籍国发生的一些突变特征的地理分布模式。
■专门针对来自低资源地区的移民人群的基于基因组的分子监测可能有助于预测与SARS-CoV-2变体或其他新兴病原体相关的新流行病学情景。以及为疫苗接种策略的更新提供信息。
UNASSIGNED: The implementation genomic-based surveillance on emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in low-income countries, which have inadequate molecular and sequencing capabilities and limited vaccine storage, represents a challenge for public health. To date, there is little evidence on molecular investigations of SARS-CoV-2 variants in areas where they might emerge. We report the findings of an experimental SARS-CoV-2 molecular surveillance programme for migrants, refugees, and asylum seekers arriving to Europe via Italy through the Mediterranean Sea.
UNASSIGNED: We descriptively analysed data on migrants collected at entry points in Sicily from February 2021 to May 2022. These entry points are integrated with a network of laboratories fully equipped for molecular analyses, which performed next-generation sequencing and used Nextclade and the Pangolin coronavirus disease 2019 (COVID-19) tools for clade/lineage assignment.
UNASSIGNED: We obtained 472 full-length SARS-CoV-2 sequences and identified 12 unique clades belonging to 31 different lineages. The delta variant accounted for 43.6% of all genomes, followed by clades 21D (Eta) and 20A (25.4% and 11.4%, respectively). Notably, some of the identified lineages (A.23.1, A.27, and A.29) predicted their introduction into the migration area. The mutation analysis allowed us to identify 617 different amino acid substitutions, 156 amino acid deletions, 7 stop codons, and 6 amino acid insertions. Lastly, we highlighted the geographical distribution patterns of some mutational profiles occurring in the migrants\' countries of origin.
UNASSIGNED: Genome-based molecular surveillance dedicated to migrant populations from low-resource areas may be useful for forecasting new epidemiological scenarios related to SARS-CoV-2 variants or other emerging pathogens, as well as for informing the updating of vaccination strategies.