大米,全球范围内的关键主食,由于非生物和生物应激源的流行率上升,在产量保存方面面临不断升级的挑战,近年来频繁的气候波动加剧了。此外,中国南方水稻种植区普遍的炎热气候给优质水稻的种植带来了障碍,重粮品种。解决这一困境需要开发能够承受多种压力因素的弹性品种。为了实现这一目标,我们的研究采用了广谱防爆线Digu,褐飞虱(BPH)抗性系ASD7,重粒骨干恢复系Fuhui838(FH838)和Shuhui527(SH527)作为亲本材料进行杂交和多次杂交。分子标记的掺入促进了六个靶基因的快速金字塔化(Pi5,Pita,Pid2、Pid3、Bph2和Wxb)。通过包括抗爆性的综合评价,耐BPH,耐寒性,颗粒外观,和质量,除了农艺性状选择,一条有前途的修复线,贵汇5501(GH5501),开发成功。它展示了对爆炸的广谱抗性,对75个人工接种的分离株表现出77.33%的抗性频率,对BPH的中等抗性(3.78级),幼苗期(1.80级)耐寒性强,和具有良好谷物品质的重谷物(1,000粒重,达到35.64g)的特征。稻米的主要品质参数为GH5501,除碱铺展值外,达到或超过优质食用水稻品种的二级国家标准,标志着南方水稻种植区优质重粒品种生产的显著进步。利用GH5501,一种名为Nayou5501的混合组合,具有高产率,质量很好,和对多种应力的抵抗力,2021年在广西选育并获得水稻品种批准。此外,基因芯片的基因组分析显示,GH5501拥有额外的20个特殊等位基因,如NRT1.1B高效利用氮,SKC1耐盐性,和STV11对水稻条纹病毒的抗性。因此,恢复系GH5501可以作为后续高产育种的宝贵资源,质量很好,和耐逆性杂交水稻品种。
Rice, a critical staple on a global scale, faces escalating challenges in yield preservation due to the rising prevalence of abiotic and biotic stressors, exacerbated by frequent climatic fluctuations in recent years. Moreover, the scorching climate prevalent in the rice-growing regions of South China poses obstacles to the cultivation of good-quality, heavy-grain varieties. Addressing this dilemma requires the development of resilient varieties capable of withstanding multiple stress factors. To achieve this objective, our study employed the broad-spectrum blast-resistant line Digu, the brown planthopper (BPH)-resistant line ASD7, and the heavy-grain backbone restorer lines Fuhui838 (FH838) and Shuhui527 (SH527) as parental materials for hybridization and multiple crossings. The incorporation of molecular markers facilitated the rapid pyramiding of six target genes (Pi5, Pita, Pid2, Pid3, Bph2, and Wxb ). Through a comprehensive evaluation encompassing blast resistance, BPH resistance, cold tolerance, grain appearance, and quality, alongside agronomic trait selection, a promising restorer line, Guihui5501 (GH5501), was successfully developed. It demonstrated broad-spectrum resistance to blast, exhibiting a resistance frequency of 77.33% against 75 artificially inoculated isolates, moderate resistance to BPH (3.78 grade), strong cold tolerance during the seedling stage (1.80 grade), and characteristics of heavy grains (1,000-grain weight reaching 35.64 g) with good grain quality. The primary rice quality parameters for GH5501, with the exception of alkali spreading value, either met or exceeded the second-grade national standard for premium edible rice varieties, signifying a significant advancement in the production of good-quality heavy-grain varieties in the southern rice-growing regions. Utilizing GH5501, a hybrid combination named Nayou5501, characterized by high yield, good quality, and resistance to multiple stresses, was bred and received approval as a rice variety in Guangxi in 2021. Furthermore, genomic analysis with gene chips revealed that GH5501 possessed an additional 20 exceptional alleles, such as NRT1.1B for efficient nitrogen utilization, SKC1 for salt tolerance, and STV11 for resistance to rice stripe virus. Consequently, the restorer line GH5501 could serve as a valuable resource for the subsequent breeding of high-yielding, good-quality, and stress-tolerant hybrid rice varieties.