背景:最近在撒哈拉以南非洲爆发的埃博拉病毒病(EVD)和马尔堡病毒病(MVD)表明需要更好地了解动物水库,疾病负担,和人类传播丝状病毒。该方案概述了系统的文献综述,以评估撒哈拉以南非洲地区感染人类的丝状病毒的患病率。次要目的是定性地描述和评估用于评估患病率的测定。
方法:本系统评价的数据源包括PubMed,Embase,和WebofScience。标题,摘要,全文将由一名主要审稿人审核,然后由一组次要审稿人审核,和数据将使用预先指定和试点的数据提取表单进行提取。审查将包括人体横断面研究,队列研究,以及直到2024年3月13日在撒哈拉以南非洲进行的随机对照试验,这些试验已经发表在同行评审的科学期刊上,没有语言限制。患病率将按病原体分层,人口,分析,和抽样方法,并在森林地块中呈现,估计患病率和95%置信区间。如果一个阶层中有足够的研究,将计算I2统计数据(使用R统计软件),如果异质性较低,数据将被汇集。此外,用于检测感染的分析将被评估。纳入审查的所有研究将使用JBI患病率关键评估工具评估质量和偏倚风险,并使用等级确定性评级评估确定性。
结论:使用患病率准确测量撒哈拉以南非洲感染人类的丝状病毒的暴露率提供了对自然史的基本理解,传输,以及亚临床感染的作用。本系统综述将确定研究差距,并为寻求提高我们对丝状病毒感染的理解的未来研究提供方向。了解自然历史,传输,亚临床感染的作用对于预测干预对疾病负担的影响至关重要。
背景:根据PRISMA-P方法中概述的指南,该协议于2023年4月7日在PROSPERO注册(ID:CRD42023415358).
BACKGROUND: Recent outbreaks of Ebola virus disease (EVD) and Marburg virus disease (MVD) in sub-Saharan Africa illustrate the need to better understand animal reservoirs, burden of disease, and human transmission of filoviruses. This protocol outlines a systematic literature review to assess the prevalence of filoviruses that infect humans in sub-Saharan Africa. A secondary aim is to qualitatively describe and evaluate the assays used to assess prevalence.
METHODS: The data sources for this systematic review include PubMed, Embase, and Web of Science. Titles, abstracts, and full texts will be reviewed for inclusion by a primary reviewer and then by a team of secondary reviewers, and data will be extracted using a pre-specified and piloted data extraction form. The review will include human cross-sectional studies, cohort studies, and randomized controlled trials conducted in sub-Saharan Africa up until March 13, 2024 that have been published in peer-reviewed scientific journals, with no language restrictions. Prevalence will be stratified by pathogen, population, assay, and sampling methodology and presented in forest plots with estimated prevalence and 95% confidence intervals. If there are enough studies within a stratum, I2 statistics will be calculated (using R statistical software), and data will be pooled if heterogeneity is low. In addition, assays used to detect infection will be evaluated. All studies included in the review will be assessed for quality and risk of bias using the JBI Prevalence Critical Appraisal Tool and for certainty using the GRADE certainty ratings.
CONCLUSIONS: Accurately measuring the rate of exposure to filoviruses infecting humans in sub-Saharan Africa using prevalence provides an essential understanding of natural history, transmission, and the role of subclinical infection. This systematic review will identify research gaps and provide directions for future research seeking to improve our understanding of filovirus infections. Understanding the natural history, transmission, and the role of subclinical infection is critical for predicting the impact of an intervention on disease burden.
BACKGROUND: In accordance with the guidelines outlined in the PRISMA-P methodology, this protocol was registered with PROSPERO on April 7, 2023 (ID: CRD42023415358).