遗传和突变是影响葡萄酚类成分的重要因素。为了研究葡萄和葡萄酒中多酚化合物的品种间和品种内差异,连续两年研究了8个葡萄品种的27个克隆。共24种多酚(9种花色苷,三种黄烷醇,五种黄酮醇,和七个酚酸)进行了分析,并测定了葡萄和葡萄酒的理化参数。多酚谱显示显著的品种和克隆多态性,和malvidin-3-O-葡萄糖苷,牡丹苷-3-O-葡萄糖苷,使用正交偏最小二乘判别分析时,表儿茶素被确定为区分不同葡萄和葡萄酒的关键生物标志物。进一步的多变量分析将这些基因型分为三个亚类,和“马尔贝克”的体细胞变体,MBVCR6具有与可滴定酸含量相关的最丰富的多酚化合物。目前的结果表明,品种和克隆变异对于获得多酚含量高的葡萄酒很重要。
Inheritance and mutations are important factors affecting grape phenolic composition. To investigate the inter- and intra-varietal differences in polyphenolic compounds among grapes and wines, 27 clones belonging to eight varieties of Vitis vinifera L. were studied over two consecutive years. A total of 24 polyphenols (nine anthocyanins, three flavanols, five flavonols, and seven phenolic acids) were analyzed, and the physicochemical parameters of the grapes and wines were determined. Polyphenol profiles showed significant varietal and clonal polymorphisms, and malvidin-3-O-glucoside, peonidin-3-O- glucoside, and epicatechin were identified as key biomarkers distinguishing different grapes and wines when using an orthogonal partial least squares discriminant analysis. Further multivariate analysis classified these genotypes into three subclasses, and a somatic variant of \'Malbec\', MBVCR6, had the most abundant polyphenolic compounds that were related to the titratable acid content. The current results reveal that varietal and clonal variations are important for obtaining wines with high polyphenol content.