恶性中央气道狭窄采用气道支架置入治疗,但安置后的微生物特征仍不清楚。我们研究了60名患者支架置入后的微生物特征,关注肉芽组织增殖过程中的变化。支架前采集样品(N=29),第3天支架后(N=20),和肉芽组织形成后(AS-GTF,N=43)。宏基因组测序显示呼吸道微生物区系随肉芽组织的显著变化。微生物群组成,以放线菌为主,Firmicutes,和变形杆菌,在群体中相似。在物种层面,AS-GTF组表现出显著差异,富含口臭链球菌和木氧化嗜铬杆菌。根据气管食管瘘的存在进行分析,确定连翘和嗜麦芽窄食单胞菌是主要的差异物种,富含瘘管亚组。病毒和真菌检测显示人类γ疱疹病毒4型和白色念珠菌为主要种,分别。这些发现强调了支架置入后微生物群的变化,可能与肉芽组织增生有关,告知支架放置治疗和抗感染治疗的优化。
目的:恶性中央气道狭窄是一种危及生命的疾病,可以通过气道支架置入有效治疗。然而,尽管它在临床上很重要,支架插入后呼吸道的微生物特征仍然知之甚少。本研究通过研究支架置入后恶性中央气道狭窄患者的微生物特征来解决这一差距。特别关注肉芽组织增殖过程中的微生物变化。研究结果揭示了在放置恶性中央气道支架后,呼吸道微生物群的多样性和结构发生了显着变化。值得注意的是,某些细菌种类,包括口腔消化链球菌和木氧化嗜铬杆菌,在支架后肉芽组织形成组中表现出不同的模式。此外,气管食管瘘的存在进一步影响微生物组成。这些见解为优化支架置入治疗和加强临床抗感染策略提供了有价值的参考。
Malignant central airway stenosis is treated with airway stent placement, but post-placement microbial characteristics remain unclear. We studied microbial features in 60 patients post-stent placement, focusing on changes during granulation tissue proliferation. Samples were collected before stent (N = 29), after stent on day 3 (N = 20), and after granulation tissue formation (AS-GTF, N = 43). Metagenomic sequencing showed significant respiratory tract microbiota changes with granulation tissue. The microbiota composition, dominated by Actinobacteria, Firmicutes, and Proteobacteria, was similar among the groups. At the species level, the AS-GTF group exhibited significant differences, with Peptostreptococcus stomatis and Achromobacter xylosoxidans enriched. Analysis based on tracheoesophageal fistula presence identified Tannerella forsythia and Stenotrophomonas maltophilia as the main differential species, enriched in the fistula subgroup. Viral and fungal detection showed Human gammaherpesvirus 4 and Candida albicans as the main species, respectively. These findings highlight microbiota changes after stent placement, potentially associated with granulation tissue proliferation, informing stent placement therapy and anti-infective treatment optimization.
OBJECTIVE: Malignant central airway stenosis is a life-threatening condition that can be effectively treated with airway stent placement. However, despite its clinical importance, the microbial characteristics of the respiratory tract following stent insertion remain poorly understood. This study addresses this gap by investigating the microbial features in patients with malignant central airway stenosis after stent placement, with a specific focus on microbial changes during granulation tissue proliferation. The findings reveal significant alterations in the diversity and structure of the respiratory tract microbiota following the placement of malignant central airway stents. Notably, certain bacterial species, including Peptostreptococcus stomatis and Achromobacter xylosoxidans, exhibit distinct patterns in the after-stent granulation tissue formation group. Additionally, the presence of tracheoesophageal fistula further influences the microbial composition. These insights provide valuable references for optimizing stent placement therapy and enhancing clinical anti-infective strategies.