保护和治疗抗炎和抗氧化能力的Malapteruruselectricus(F.Malapteruridae)鱼皮甲醇提取物(FE)(300mg/kg.b.wt/天,持续7天,口服)在尿酸单钠(MSU)诱导的关节炎Wistar白化病雄性大鼠关节中进行了测试。血清尿酸,TNF-α,IL-1β,NF-?B,MDA,GSH,过氧化氢酶,SOD,和谷胱甘肽还原酶水平都被测量。根据调查结果,FE在保护组和治疗组均显着降低了尿酸水平和踝关节肿胀。此外,它通过下调炎症细胞因子而具有抗炎作用,主要通过减少氧化应激和增加抗氧化状态。在用FE保护和治疗的大鼠中,所有上述病变均显着改善,根据组织病理学发现。iNOS免疫染色显示,用FE保护和治疗的关节炎大鼠具有弱阳性免疫反应细胞。植物化学分析表明,FE富含脂肪酸和氨基酸。最丰富的化合物是疫苗(24.52%),9-十八碳烯(11.66%),棕榈(34.66%),硬脂酸(14.63%),甘氨酸(0.813毫克/100毫克),和丙氨酸(1.645毫克/100毫克)。广泛的分子建模和动力学模拟实验表明,化合物4具有靶向和抑制COX-2同工型的潜力,对COX-2具有更高的亲和力。因此,我们认为FE可能是关节炎的一个有前途的保护和治疗选择,有助于这种慢性炎症性疾病的预防和进展。
The protective and therapeutic anti-inflammatory and antioxidant potency of Malapterurus electricus (F. Malapteruridae) skin fish methanolic extract (FE) (300 mg/kg.b.wt/day for 7 days, orally) was tested in monosodium urate(MSU)-induced arthritic Wistar albino male rats\' joints. Serum uric acid, TNF-α, IL-1β, NF-𝜅B, MDA, GSH, catalase, SOD, and glutathione reductase levels were all measured. According to the findings, FE significantly reduced uric acid levels and ankle swelling in both protective and therapeutic groups. Furthermore, it has anti-inflammatory effects by downregulating inflammatory cytokines, primarily through decreased oxidative stress and increased antioxidant status. All the aforementioned lesions were significantly improved in protected and treated rats with FE, according to histopathological findings. iNOS immunostaining revealed that protected and treated arthritic rats with FE had weak positive immune-reactive cells. Phytochemical analysis revealed that FE was high in fatty and amino acids. The most abundant compounds were vaccenic (24.52%), 9-octadecenoic (11.66%), palmitic (34.66%), stearic acids (14.63%), glycine (0.813 mg/100 mg), and alanine (1.645 mg/100 mg). Extensive molecular modelling and dynamics simulation experiments revealed that compound 4 has the potential to target and inhibit COX isoforms with a higher affinity for COX-2. As a result, we contend that FE could be a promising protective and therapeutic option for arthritis, aiding in the prevention and progression of this chronic inflammatory disease.