Entamoeba物种感染人类和非人灵长类动物,引起与潜在的人畜共患传播相关的担忧。因此,人类内阿米巴感染的流行对其在地区的管理至关重要,猕猴的感染率很高。以前,我们证明了加德满都恒河猴中普遍存在的纳塔利大肠杆菌感染,尼泊尔。在这项研究中,我们调查了野生恒河猴访问过的两所学校的185名学童中的Entamoeba感染情况,以评估其传播风险.对Entamoeba物种的基于PCR的筛选鉴定了13%的大肠杆菌和0.5%的人粪便样品中的E.dispar。然而,纳塔利大肠杆菌和查顿尼大肠杆菌感染,在猕猴中普遍存在,在人类样本中没有检测到。这表明Entamoebaspp。在学校环境中不会通过猕猴传播。我们调查了住在学校附近寺庙的恒河猴,以及居住在ShivapriNagarjun国家公园的恒河猴和阿萨姆猕猴,加德满都.在49个猕猴粪便样本中,E.Chattoni,大肠杆菌,E.Nuttalli,和E.dispar检测到92%,86%,41%,还有18%的样本,分别。值得注意的是,猕猴的大肠杆菌感染主要在寺庙中流行。从Nagarujun分离的样品在两个tRNA连接的短串联重复位点上显示出与从人类分离的E.dispar相同的基因型,提示人类向猕猴的潜在传播.从三个地点的猕猴中获得的培养的Nuttalli菌株的基因型分析表明,地理距离而不是猕猴物种的差异在寄生虫的遗传多样性中起着至关重要的作用。纳塔利大肠杆菌菌株的系统发育树,包括以前分离的菌株,反映了隔离地点的地理分布。这项研究揭示了猕猴和人类中Entamoeba传播和遗传多样性的复杂动态。
Entamoeba species infect humans and non-human primates, raising concerns associated with potential zoonotic transmission. Therefore, the prevalence of human Entamoeba infections is crucial for its management in areas, where
macaques exhibit high infection rates. Previously, we demonstrated prevalent E. nuttalli infections in rhesus
macaques in Kathmandu, Nepal. In this study, we surveyed Entamoeba infection among 185 schoolchildren from two schools visited by wild rhesus macaques to assess the risk of transmission. PCR-based screening for Entamoeba species identified E. coli in 13 % and E. dispar in 0.5 % of the human stool samples. However, E. nuttalli and E. chattoni infections, prevalent in macaques, were not detected in human samples. This suggests that Entamoeba spp. are not transmitted through
macaques in the school environment. We surveyed the rhesus
macaques living in the temple near schools as well as the rhesus and Assam
macaques inhabiting Shivapri Nagarjun National Park, Kathmandu. Among the 49 macaque stool samples, E. chattoni, E. coli, E. nuttalli, and E. dispar were detected in 92 %, 86 %, 41 %, and 18 % of the samples, respectively. Notably, E. dispar infections in
macaques were mostly prevalent in the temple. A sample isolated from Nagarujun showed an identical genotype at two tRNA-linked short tandem repeat loci to that of E. dispar isolated from humans, suggesting potential transmission from humans to macaques. Genotypic analysis of cultured E. nuttalli strains obtained from the macaques colonizing three locations demonstrated that the geographical distance rather than differences in macaque species played a crucial role in the genetic diversity of the parasites. The phylogenetic tree of E. nuttalli strains, including the previously isolated strains, reflected the geographical distribution of the isolation sites. This study sheds light on the intricate dynamics of Entamoeba transmission and genetic diversity in macaques and humans.