MYB转录因子调节植物生长,发展,和防御反应。然而,有关Ipomoea物种中MYB基因家族的信息很少。在这里,我们对该基因家族进行了全面的全基因组比较分析,甘薯(I.batatas),I.Trifida,I.triloba,一、无,我是紫癜,I.Cairica,和I.水生,并鉴定出296、430、411、291、226、281和277个MYB基因,分别。确定的MYB基因分为五种类型:1R-MYB(MYB相关),2R-MYB(R2R3-MYB),3R-MYB(R1R2R3-MYB),4R-MYB,和5R-MYB,MYB相关或R2R3-MYB类型是7个物种中最丰富的MYB基因。根据拟南芥中MYB超家族的系统发育拓扑和分类,将IpomoeaMYB基因分为不同的亚组。对基因结构和蛋白质基序的分析表明,同一系统发育组中的成员呈现相似的外显子/内含子和基序组织。鉴定出的MYB基因不均匀地定位在每个Ipomoea物种的染色体上。重复分析表明,分段和串联重复有助于扩展IpomoeaMYB基因。非同义替换(Ka)到同义替换(Ks)[Ka/Ks]分析表明,重复的IpomoeaMYB基因主要在纯化选择中。在MYB启动子中检测到许多与应激反应相关的顺式调节元件。分析了六个涉及非生物和生物胁迫的甘薯转录组数据集,和MYB不同的表达基因对应激处理的反应被检测到。此外,选择10个甘薯MYBDEGs进行qRT-PCR分析。结果表明,有四个对生物胁迫有反应(茎线虫和毛毛虫病原体感染),有六个对生物胁迫有反应(寒冷,干旱,和盐)。该结果可能为Ipomoea基因组中MYB基因的进化提供新的见解,并有助于未来甘薯的分子育种。
The MYB transcription factors regulate plant growth, development, and defense responses. However, information about the MYB gene family in Ipomoea species is rare. Herein, we performed a comprehensive genome-wide comparative analysis of this gene family among seven Ipomoea species, sweet potato (I. batatas), I. trifida, I. triloba, I. nil, I. purpurea, I. cairica, and I. aquatic, and identified 296, 430, 411, 291, 226, 281, and 277 MYB genes, respectively. The identified MYB genes were classified into five types: 1R-MYB (MYB-related), 2R-MYB (R2R3-MYB), 3R-MYB (R1R2R3-MYB), 4R-MYB, and 5R-MYB, and the MYB-related or R2R3-MYB type was the most abundant MYB genes in the seven species. The Ipomoea MYB genes were classed into distinct subgroups based on the phylogenetic topology and the classification of the MYB superfamily in Arabidopsis. Analysis of gene structure and protein motifs revealed that members within the same phylogenetic group presented similar exon/intron and motif organization. The identified MYB genes were unevenly mapped on the chromosomes of each Ipomoea species. Duplication analysis indicated that segmental and tandem duplications contribute to expanding the Ipomoea MYB genes. Non-synonymous substitution (Ka) to synonymous substitution (Ks) [Ka/Ks] analysis showed that the duplicated Ipomoea MYB genes are mainly under purifying selection. Numerous cis-regulatory elements related to stress responses were detected in the MYB promoters. Six sweet potato transcriptome datasets referring to abiotic and biotic stresses were analyzed, and MYB different expression genes\' (DEGs\') responses to stress treatments were detected. Moreover, 10 sweet potato MYB DEGs were selected for qRT-PCR analysis. The results revealed that four responded to biotic stress (stem nematodes and Ceratocystis fimbriata pathogen infection) and six responded to the biotic stress (cold, drought, and salt). The results may provide new insights into the evolution of MYB genes in the Ipomoea genome and contribute to the future molecular breeding of sweet potatoes.