UNASSIGNED: Actinobacillus pleuropneumoniae (APP) is a serious pathogen that affects the development of livestock breeding. Due to excessive use of antimicrobial drugs, many multidrug-resistant bacteria have emerged and spread, which have threatened the livestock industry. Therefore, we established a peristaltic pump infection model (PPIM) to evaluate the susceptibility change and pharmacokinetic/pharmacodynamic (PK/PD) integration of tulathromycin against APP during the mutant selection window (MSW) for preventing the emergence of mutant-resistant bacteria.
UNASSIGNED: The 99% minimum inhibitory concentration (MIC99) and mutant prevention concentration (MPC) of tulathromycin against APP were measured using the agar-plate method. After the model of dynamic infection had been established based on tulathromycin data in lungs, different dosages were administered to make the drug concentrations located in different parts of the MSW. The population and sensitivity of APP were monitored. Tulathromycin concentrations were measured by high-performance liquid chromatography-tandem mass spectrometry. Finally, a sigmoid Emax model was used to analyze the relationships between PK/PD parameters and antibacterial effects.
UNASSIGNED: The values of MIC, MIC99, and MPC of tulathromycin against APP were 2, 1.4, and 44.8 μg/mL, respectively. The PPIM was stable. An elimination effect without regrowth was observed at 5.6 to 44.8 μg/mL (-4.48 to -7.05 Log10 CFU/mL, respectively). The MIC of APP increased 32-fold at 8 MIC99. AUC168 h/MIC99 had the best fit with the antibacterial effect (R 2 = 0.9867). The AUC168 h/MIC99 required to achieve bacteriostatic, bactericidal, and clearance effects were 1.80, 87.42, and 198 h, respectively. Our results could provide guidance for the clinical application of tulathromycin to treat APP infection and avoid the generation of drug-resistant bacteria.

This research investigates the formation mechanism of soot and particulate matter during the pyrolysis and gasification of waste derived from Municipal Solid Waste (MSW) in a laboratory scale drop tube furnace. Compared with CO2 gasification atmosphere, more ultrafine particles (PM0.2, aerodynamic diameter less than 0.2 μm) were generated in N2 atmosphere at 1200℃, which were mainly composed of polycyclic aromatic hydrocarbons (PAHs), graphitic carbonaceous soot and volatile alkali salts. High reaction temperatures promote the formation of hydrocarbon gaseous products and their conversion to PAHs, which ultimately leads to the formation of soot particles. The soot particles generated by waste derived from MSW pyrolysis and gasification both have high specific surface area and well-developed pore structure. Compared with pyrolysis, the soot generated by gasification of waste derived from MSW had smaller size and higher proportion of inorganic components. The higher pyrolysis temperature led to the collapse of the mesoporous structure of submicron particles, resulting in a decrease in total pore volume and an increase in specific surface area. Innovatively, this research provides an explanation for the effect of reaction temperature/ CO2 on the formation pathways and physicochemical properties of soot and fine particulate matter.

Inorganic chlorine is susceptible to water and soil salinization due to its non-degradability and high mobility. To clarify the environmental risks associated with the active inorganic chlorine in municipal solid waste (MSW), the specific characteristics and contributions of inorganic chlorine in different MSW categories were investigated in this study. MSW samples were collected from eight representative waste classification residential areas in Hangzhou, China. It was found that the inorganic chlorine content in different MSW categories varied significantly (0-113 mg/g). Perishable waste, paper, and plastic were found to be the main sources of inorganic chlorine in MSW. A four-category classification system was used to quantify the contribution of inorganic chlorine from each waste category. It was found that the misclassification of inorganic chlorine contributions from perishable waste and other waste accounted for 51.96% and 48.04%, respectively. However, when correctly classified into the four-category system, their contributions were reduced to 67.14% and 30.65%, respectively. Therefore, MSW classification showed a significant reduction in the overall contribution of inorganic chlorine. The misclassification reduces the contribution of inorganic chlorine to 48.04%, while correct classification increases the reduction to 69.35%.

Pyrolysis is a promising method for recovering resources and energy products from municipal solid waste (MSW). Predicting MSW pyrolysis products is crucial for establishing an efficient pyrolysis system for resource recovery. In this study, a database was established based on MySQL to record relevant information on MSW pyrolysis, which includes the MSW ultimate analysis results, proximate analysis results, parameters of pyrolysis operation and yields of pyrolysis products, etc. Based on the database and with help of a deep artificial neural network (ANN) which contains 10 hidden layers, a prediction model was successfully established to predict the yield of char, liquid and gas products from MSW pyrolysis. The results showed that the coefficients of determination for predicting the yields of char, liquid and gas from the MSW pyrolysis are 0.841, 0.84, and 0.85, respectively; these values demonstrate an accuracy comparable to that achieved for product prediction from single biomass, indicating a successful model performance. The results also show that ash content and temperature are the most important input factors influencing the outputs, namely, yields of char, liquid and gas. The results of this study can help to achieve a more efficient design of the pyrolysis system and improve the recovery of the desired pyrolysis products.

Aeration plays a crucial role in accelerating the secondary compression of municipal solid waste (MSW) for the scientific implementation of aerobic bioreactor technology. There are few comparative reports on the secondary compaction characteristics of MSW in aerobic and anaerobic bioreactors. In this study, six long-term compression tests were conducted to analyze the impact of aeration on MSW compression characteristics, considering two degradation conditions (i.e. aerobic and anaerobic conditions) and three overburden stresses (i.e. 30, 50 and 100 kPa). Model-fitting analysis was employed to examine the data from the tests and exiting literatures. The results showed that aeration effectively increased the rate of secondary compression, and slightly enhanced the steady-state secondary compression strain. In addition, these enhancements tended to decrease with increasing stresses. The increment ratio of the secondary compression rate constant (Rk) was concentrated in the range of 25 % to 100 %, and increases with the increase of aeration rate. The increment ratio of the steady-state secondary compression strain (Rε) ranged from 10 % to 90 %, for the MSW with higher content of paper and wood exhibited higher Rε. The advance ratio of the secondary compression stabilization time (Rt) fell within the range of 20-50 %, and Rt is higher when the moisture content is in the range of 50-65 %. These findings provide valuable guidance on the accelerated stabilization in aerobic bioreactors, providing practical references for the application of aerobic technology to informal landfills.

In the test and treat initiative, high-risk populations are screened for human immunodeficiency virus (HIV) infection and start early treatment if diagnosed positive. This study explores factors associated with willingness to initiate testing and immediate treatment among men who have sex with men (MSM) in Nairobi and its environs. The study was informed by a conceptual framework combining the AIDS Risk Reduction Model (ARRM) and the Modified Social Ecological Model.
This cross-sectional exploratory study targeted MSM (aged 18-60 years) reporting active engagement in anal or oral sex with men in Nairobi and its surrounding areas. Purposive sampling was used to identify data collection sites, and then snowballing was employed to reach the respondents. Data analysis was performed using SPSS version 23, and binary logistics regression was used for inferential analysis.
Between July 2018 and June 2019, 391 MSM were recruited to fill out a self-administered questionnaire, out of which 345 complete questionnaires were analyzed. Never been tested for HIV, private/NGO as the facility of the last HIV test, and had unprotected anal sex were listed as the reasons for taking the most recent test, and the results of the most recent HIV test and seeking a post self-test confirmation were associated with a higher likelihood of accepting the immediate HIV test and treat initiative. Additionally, a preference for a health provider as the first source of support, belief in the efficacy of ARVs, and disclosure about being on ART were the other reasons. Additionally, being aged 25+ years, having more than 60 USD monthly income, and having inconsistent condom use during sex were associated with a higher likelihood of accepting the immediate HIV test and treat initiative. Barriers to the immediate test and treat strategy included stigma from healthcare providers and concerns about disruptions in lifestyle through antiretroviral therapy (ART) use.
Interventions aimed at increasing the HIV test and treat initiative in Kenya may need to take into account the demographic and social characteristics of MSM, including age, lack of habitual HIV testing, and lifestyle changes before and upon enrollment in ART. Projects should also consider working closely with healthcare facilities to strengthen treatment preparation, especially for asymptomatic MSM and those who may not be immediately willing to choose the test and treat strategy.

Anaerobic landfills have long been the primary means of municipal solid waste (MSW) disposal in China. Landfills are the third largest emission source of methane (CH4), which is the second most abundant greenhouse gas in the atmosphere and has a high greenhouse effect. To date, there have been no reliable model predictions of long-term CH4 emissions from landfills in China. In this study, two general models, IPCC and LandGEM, were introduced to simulate CH4 emissions from all landfills in China. By comparing the results of the Shuangkou landfill in Tianjin with the default and local parameters, the local parameters were fixed to simulate landfill CH4 emissions in 31 regions over 40 years (2003-2042). The MSW landfills were obtained from statistical data for 18 years (2003-2020). The total emissions in China predicted by LandGEM and IPCC were 2.42 E+07 Mg and 2.36 E+07 Mg, respectively. These data provide a reliable reference for determining the long-term CH4 emissions from landfills in China.

The aim of this article is to examine how Life Cycle Thinking (LCT) contributes to the development of Waste Management Plans (WMPs). The case of Italy has been deeply investigated. The article first analyses whether and how the LCT methodologies were applied to the 21 regional WMPs; then, it draws indications for using LCT in the preparation of a WMP. Moreover, it outlines why the Life Cycle Assessment (LCA) methodology could be used as a powerful tool for regional planning in the waste field, analysing the indications for preparing a WMP that already exist at the European level and in the Italian National WM Programme. Results reveal that only four of the 21 regional WMPs include comprehensive and site-specific LCA studies. Building on these case study results, insights into the opportunities and benefits associated with incorporating LCT methodologies into WMP development and implementation are provided. This study underscores the critical importance of LCT and LCA in promoting sustainable waste management practices, ensuring compliance with European directives, and offering a foundation for more informed regional planning strategies.

With the development of incineration technologies, incineration has become the most common treatment method of municipal solid waste in China. However, stabilized fly ash may enter landfills during the transition from landfill to incineration, which caused uncertain impact on landfill waste stabilization. Two simulated co-landfill columns were constructed based on different co-landfill methods (layer co-landfill and mixed co-landfill) to investigate the effect of stabilized fly ash co-landfilled municipal solid waste for bacterial community succession and change in metabolic pathways during hydrolysis-acidogenesis stage. The mixed co-landfill method resulted in higher degree of organic matter degradation, and the concentrations of volatile fatty acids (VFA) and chemical oxygen demand (COD) in leachate were higher. The dominant phyla were Firmicutes in the layered co-landfill column and Bacteroidetes in mixed co-landfill column. The dominant genera for the total bacterial composition and VFA production were different, Pseudomonas and Propionibacterium, Proteiniphilum and unclassified Bacteroides were the dominant genera responsible for VFA generation in the layered and mixed co-landfill columns. The genes for butyrate production were enriched in the layered co-landfill column, whereas those related to acetate production were enriched in mixed co-landfill column. However, the layered co-landfill inhibited the microbial metabolic activity at the end of the co-landfill process.

Studies on the occurrence and fates of emerging organic micropollutants (EOMPs) like pharmaceuticals and pesticides in MSWs are scarce in the literature. Therefore, MSWs were sampled from 20 Indian landfills and characterized for five widely consumed EOMPs (chlorpyrifos, cypermethrin, carbofuran, carbamazepine, and sodium diclofenac), physicochemical, and biological properties. The pesticide (median: 0.17-0.44 mg kg-1) and pharmaceutical (median: 0.20-0.26 mg kg-1) concentrations significantly fluctuated based on landfill localities. Eventually, principal component and multi-factor (MFA) models demonstrated close interactions of EOMPs with biological (microbial biomass and humification rates) and chemical (N, P, K, Ca, S, etc.) properties of MSWs. At the same time, the MFA resolved that EOMPs\' fates in MSWs significantly differ from bigger cosmopolitan cities to smaller rural townships. Correspondingly, the concentration-driven ecological risks were high in 15 MSWs with EOMP-toxicity ranks of diclofenac > carbofuran = chlorpyrifos > cypermethrin > carbamazepine. The EOMPs\' dissolution dynamics and source apportionments were evaluated using the positive matrix factorization (PMF) model for the first time on experimental data, extracting four anthropogenic sources (households, heterogeneous business centers, agricultural, and open drains). The most significant contribution of EOMPs to MSWs was due to heterogeneous business activity. Notably, the aging of soluble chemical fractions seems to influence the source characteristics of EOMPs strongly.