ZizanialatifoliaTurcz.长期以来一直被用作东南亚的食物来源。谷物,茎,以及Z.latifolia的叶子及其主要成分,tricin,也被研究以确定它们的生物活性。以前,我们使用酶混合物水解Z.latifolia的地上部分,以最大限度地提高Z.latifolia提取物的tricin含量。然而,酶处理的Z.latifolia提取物(ETZL;DermaNiA™)的安全性尚未确定。在这项研究中,我们进行了90天的体内重复剂量评估和遗传毒性研究,以评估ETZL的毒理学潜力.EZTL在细菌回复突变试验中没有表现出遗传毒性,体外染色体畸变试验,或体内微核试验。此外,在接受高剂量EZTL治疗(5000mg/kg体重(bw)/天)90天,恢复期为4周,雄性或雌性大鼠体重或血液学和血清生物学参数没有变化.前胃有明显变化,肾脏,测试组中的肾上腺,但是这些变化,或恢复的趋势,在恢复组未观察到。基于这些数据,在大鼠中,无不良反应水平确定为1250mg/kgbw/天。
Zizania latifolia Turcz. has long been used as a food source in Southeast Asia. The grains, stems, and leaves of Z. latifolia and its major component, tricin, have also been studied to determine their biological activities. Previously, we hydrolyzed the aerial part of Z. latifolia using an enzyme mixture to maximize the tricin content of the Z. latifolia extract. However, the safety of enzyme-treated Z. latifolia extract (ETZL; DermaNiA™) has not yet been determined. In this study, we performed an in vivo 90-day repeated-dose evaluation and genotoxicity study to assess the toxicological potential of ETZL. EZTL did not exhibit genotoxicity in the bacterial reverse mutation test, in vitro chromosomal aberration assay, or in vivo micronucleus test. Moreover, no changes in body weight or hematological and serum biological parameters were observed in male or female rats under high-dose EZTL treatment (5000 mg/kg body weight (bw)/day) for 90 days with a 4-week recovery period. Significant changes were noted in the forestomach, kidneys, and adrenal glands in the test groups, but these changes, or tendency for recovery, were not observed in the recovery group. Based on these data, the no adverse effect level was determined to be 1250 mg/kg bw/day in rats.