环状RNA(circularRNAs)的异常表达与多囊卵巢综合征(PCOS)的进展有关,这通常会导致女性不孕。在这项研究中,我们确定了circ_0030018在PCOS中的作用。定量聚合酶链反应(qPCR)检测circ_0030018、microRNA(miR)-136、迁移和侵袭增强子1(MIEN1)的表达水平。进行细胞计数试剂盒-8和5-乙炔基-2'-脱氧尿苷测定以分析KGN细胞的增殖。使用荧光激活的细胞分选分析细胞凋亡。进行Transwell测定以测量细胞的迁移和侵袭能力。qPCR和Western印迹用于测量E-cadherin的水平,N-钙黏着蛋白,蜗牛,还有波形蛋白.circ_0030018或MIEN1表达与miR-136表达的相关性通过荧光素酶报告基因和RNA下拉测定来证实。结果显示circ_0030018在PCOS和KGN细胞中表达上调。circ_0030018的击倒抑制了增殖,迁移,和细胞的入侵,同时促进其凋亡。circ_0030018海绵化miR-136,其靶向MIEN1。此外,miR-136的下调消除了circ_0030018沉默的影响,而MIEN1的过表达逆转了miR-136对KGN细胞的诱导作用。总之,circ_0030018的缺失通过miR-136/MIEN1轴延迟了PCOS的进展。
The abnormal expression of circular RNAs (circRNAs) is associated with the progression of polycystic ovary syndrome (PCOS), which commonly causes infertility in women. In this study, we identified the role of circ_0030018 in PCOS. Quantitative polymerase chain reaction (qPCR) was used to detect the expression levels of circ_0030018, microRNA (miR)-136, and migration and invasion enhancer 1 (
MIEN1). Cell counting kit-8 and 5-ethynyl-2\'-deoxyuridine assays were performed to analyze the proliferation of KGN cells. Apoptosis was analyzed using fluorescence-activated cell sorting. Transwell assays were performed to measure the migration and invasion abilities of cells. qPCR and Western blotting were used to measure the levels of E-cadherin, N-cadherin, Snail, and vimentin. The correlation of circ_0030018 or
MIEN1 expression with miR-136 expression was confirmed via luciferase reporter and RNA pull-down assays. Results showed that circ_0030018 expression was upregulated in patients with PCOS and KGN cells. Knockdown of circ_0030018 suppressed the proliferation, migration, and invasion of cells, while promoting their apoptosis. circ_0030018 sponged miR-136, which targeted
MIEN1. Moreover, downregulation of miR-136 abrogated the effects of circ_0030018 silencing, while the overexpression of
MIEN1 reversed the miR-136-induced effect on KGN cells. In summary, loss of circ_0030018 delayed the progression of PCOS via the miR-136/
MIEN1 axis.