红木无名指1(MGRN1),E3泛素,参与几个生理和神经病理学过程。尽管mgrn1mRNA广泛分布于中枢神经系统(CNS),有关其细胞和亚细胞定位的详细信息尚不清楚,其生理作用尚不清楚。在这项研究中,我们的目的是使用新产生的针对MGRN1的抗体来确定MGRN1在小鼠CNS中的分布.我们发现MGRN1蛋白在大多数神经元细胞体中表达。在中枢神经系统不同区域的灰质神经纤维中也观察到强烈的MGRN1表达,包括主嗅觉灯泡,大脑皮层,尾状,壳核,丘脑核,下丘脑核,内侧隆起,上丘,海马体,齿状回,和脊髓。相反,在胶质细胞中未观察到MGRN1表达。双重荧光和免疫电子显微镜分析显示MGRN1在突触前和神经元线粒体外膜附近的细胞内分布。这些发现表明MGRN1在整个CNS中更广泛地表达;此外,MGRN1的细胞内表达提示其可能在突触和线粒体功能中起重要作用。
Mahogunin ring finger 1 (
MGRN1), an E3 ubiquitin, is involved in several physiological and neuropathological processes. Although
mgrn1 mRNA is widely distributed in the central nervous system (CNS), detailed information on its cellular and subcellular localization is lacking and its physiological role remains unclear. In this study, we aimed to determine the distribution of MGRN1 in the mouse CNS using a newly produced antibody against
MGRN1. We found that the
MGRN1 protein was expressed in most neuronal cell bodies. An intense MGRN1 expression was also observed in the neuropil of the gray matter in different regions of the CNS, including the main olfactory bulb, cerebral cortex, caudate, putamen, thalamic nuclei, hypothalamic nuclei, medial eminence, superior colliculus, hippocampus, dentate gyrus, and spinal cord. Contrastingly, no
MGRN1 expression was observed in glial cells. Double fluorescence and immunoelectron microscopic analyses revealed the intracellular distribution of MGRN1 in pre-synapses and near the outer membrane of the mitochondria in neurons. These findings indicate that MGRN1 is more widely expressed throughout the CNS; additionally, the intracellular expression of MGRN1 suggests that it may play an important role in synaptic and mitochondrial functions.