背景:口腔鳞状细胞癌(OSCC)是头颈部鳞状细胞癌中最常见的癌症之一。口腔癌及其晚期出现的频率不断增加是全球主要的健康问题。MicroRNAs(miRNAs)在癌症的生长和进展中发挥重要作用。根据不断增长的数据,而没有关于miR-7113-3p和miR-6721-5p参与OSCC的信息。在这篇文章中,MAP2K1,miR-7113-3p,和miR-6721-5p在口腔鳞状细胞癌进展中可能的生物学功能。
方法:我们使用定量实时PCR(检测MAP2K1,miR-7113-3p的mRNA表达,和miR-6721-5p在来自30例患者的新鲜冷冻OSCC组织和邻近的新鲜冷冻组织中,我们调查了它们与临床参数的关系。
结果:MAP2K1在肿瘤组织中的表达明显高于正常组织,而miR7113-3p和miR-6721-5p表达显著降低。此外,在MAP2K1表达增加和神经周浸润之间也观察到P=0.04的统计学相关性。此外,我们注意到miR-7113-3p的下调似乎与MAP2K1的过表达呈正相关(P=0.0218),miR-6721-5p的下调与MAP2K1的过表达呈负相关(P=0.7771)。
结论:基于这些发现,miR-7113-3p和miR-6721-5p可能是OSCC患者的前瞻性生物标志物,并可用于早期检测OSCC,用于未来的诊断。MAP2K1过表达与OSCC的发展和神经周侵袭有关。
BACKGROUND: Oral squamous cell carcinoma (OSCC) is one of the most common cancers in the head and neck squamous cell cancer group. The increasing frequency of oral carcinomas and their late-stage appearance is a major worldwide health concern. MicroRNAs (miRNAs) appear to play an important role in cancer growth and progression, according to growing data, whereas no information is available regarding miR-7113-3p and miR-6721-5p involvement in OSCC. In this article, the expression of MAP2K1, miR-7113-3p, and miR-6721-5p was examined for possible bio-logical functions in the advancement of oral squamous cell carcinoma.
METHODS: We used quantitative real-time PCR (to examine the mRNA expression of MAP2K1, miR-7113-3p, and miR-6721-5p in fresh frozen OSCC tissues and adjacent normal fresh frozen tissues from 30 patients, and we investigated their relationship with clinical parameters.
RESULTS: MAP2K1 expression was found to be dramatically increased in tumor tissues than in normal tissues, whereas miR7113-3p and miR-6721-5p expression was significantly decreased. Furthermore, a statistical correlation of P = 0.04 was also observed between increased MAP2K1 expression and perineural invasion. Additionally, we noted that the downregulation of miR-7113-3p appears to correlate positively with overexpression of MAP2K1 (P = 0.0218), and a negative correlation was observed between downregulation of miR-6721-5p and overexpression of MAP2K1 (P = 0.7771).
CONCLUSIONS: Based on these findings, miR-7113-3p and miR-6721-5p might be prospective bio-markers for OSCC patients, and could be utilized to detect OSCC at an early stage for future dia-gnosis. MAP2K1 overexpression has been linked to the development of OSCC and perineural invasion.