背景:忍冬.在中药中广泛使用。药用L.japonica主要由干燥的花蕾和部分开放的花组成,因此花卉是重要的质量指标。MADS-box基因编码调节花发育的转录因子。然而,对L.japonica中的这些基因知之甚少。
结果:在这项研究中,48个MADS-box基因在日本血吸虫中被鉴定出来,包括20个I型基因(8个Mα,2Mβ,和10个Mγ)和28个II型基因(26个MIKCc和2个MIKC*)。I型和II型基因在基因结构上有显著差异,保守域,蛋白质结构,染色体分布,系统发育,和表达模式。I型基因的基因结构更简单,缺少K域,蛋白质结构保守性低,串联分布在染色体上,有更频繁的谱系特异性重复,并以低水平表示。相比之下,II型基因具有更复杂的基因结构;包含保守的M,I,K,和C结构域;具有高度保守的蛋白质结构;并且在整个开花期都以高水平表达。与拟议的拟南芥ABCDE花器官身份确定模型直系同源的11个花同源MADS-box基因,在L.japonica中鉴定。通过整合这些基因的表达模式和蛋白质相互作用数据,我们开发了一种可能的花器官身份确定模型。
结论:本研究基因组广泛地鉴定和表征了日本血吸虫中的MADS-box基因家族。鉴定了11种花同源MADS-box基因,并开发了一种可能的花器官鉴定模型。这项研究有助于我们了解MADS-box基因家族及其可能参与日本羊角花器官发育的情况。
BACKGROUND: Lonicera japonica Thunb. is widely used in traditional Chinese medicine. Medicinal L. japonica mainly consists of dried flower buds and partially opened flowers, thus flowers are an important quality indicator. MADS-box genes encode transcription factors that regulate flower development. However, little is known about these genes in L. japonica.
RESULTS: In this study, 48 MADS-box genes were identified in L. japonica, including 20 Type-I genes (8 Mα, 2 Mβ, and 10 Mγ) and 28 Type-II genes (26 MIKCc and 2 MIKC*). The Type-I and Type-II genes differed significantly in gene structure, conserved domains, protein structure, chromosomal distribution, phylogenesis, and expression pattern. Type-I genes had a simpler gene structure, lacked the K domain, had low protein structure conservation, were tandemly distributed on the chromosomes, had more frequent lineage-specific duplications, and were expressed at low levels. In contrast, Type-II genes had a more complex gene structure; contained conserved M, I, K, and C domains; had highly conserved protein structure; and were expressed at high levels throughout the flowering period. Eleven floral homeotic MADS-box genes that are orthologous to the proposed Arabidopsis ABCDE model of floral organ identity determination, were identified in L. japonica. By integrating expression pattern and protein interaction data for these genes, we developed a possible model for floral organ identity determination.
CONCLUSIONS: This study genome-widely identified and characterized the MADS-box gene family in L. japonica. Eleven floral homeotic MADS-box genes were identified and a possible model for floral organ identity determination was also developed. This study contributes to our understanding of the MADS-box gene family and its possible involvement in floral organ development in L. japonica.