白细胞介素-1β(IL1β)主要由外周和脑中的单核细胞产生。然而,迄今为止,已使用血浆或血清测定对重度抑郁症(MDD)和抗抑郁药反应中的IL1β蛋白水平进行了研究,尽管结果相互矛盾。尽管在其他情况下广泛使用,但缺乏单核细胞测定。在这项试点研究中,我们比较评估了抑郁症MDD患者单核裂解物和血浆中的IL1β,超过治疗和健康对照(HC)。我们招募了31名连续成年MDD住院患者和25名年龄匹配的HC,性别,BMI。26名患者在治疗下完成了8周的随访。在基线(T0)和研究结束(T1)以及HC中测量患者的裂解物和血浆中的IL1β。我们计算裂解物和血浆的ΔIL1β(%)为从T0到T1的IL1β百分比变化。17名患者(65.4%的完成者)在T1时是应答者,基线BMI低于非应答者(p=0.029)。来自血浆或裂解物的基线IL1β无法有效区分抑郁症患者和HC,或在响应者和非响应者之间。然而,两个反应组在裂解物中显示出相反的IL1β轨迹,但在血浆测定中没有(反应组在时间相互作用上,p分别=0.005和0.96)。裂解物中的ΔIL1β(%)预测反应(p=0.025,AUC=0.81;准确度=84.6%)优于血浆中的ΔIL1β(%)(p=0.77,AUC=0.52),并且对于调整BMI是稳健的。总之,单核裂解物中的ΔIL1β(%)可能是抗抑郁反应的纵向生物标志物,可能有助于避免抗抑郁药的不合时宜的转换,因此需要进一步调查。
Interleukin-1 beta (IL1β) is primarily produced by monocytes in the periphery and the brain. Yet, IL1β protein levels have to date been investigated in major depressive disorder (MDD) and antidepressant response using either plasma or serum assays although with contradictory results, while mononuclear cell assays are lacking despite their extensive use in other contexts. In this pilot study, we comparatively assessed IL1β in mononuclear
lysates and plasma in depressed MDD patients over treatment and healthy controls (HC). We recruited 31 consecutive adult MDD inpatients and 25 HC matched on age, sex, and BMI. Twenty-six patients completed an 8-week follow-up under treatment. IL1β was measured in both
lysates and plasma in patients at baseline (T0) and at study end (T1) as well as in HC. We calculated ΔIL1β(%) for both
lysates and plasma as IL1β percent changes from T0 to T1. Seventeen patients (65.4% of completers) were responders at T1 and had lower baseline BMI than non-responders (p = 0.029). Baseline IL1β from either plasma or lysates could not efficiently discriminate between depressed patients and HC, or between responders and non-responders. However, the two response groups displayed contrasting IL1β trajectories in
lysates but not in plasma assays (response group by time interactions, p = 0.005 and 0.96, respectively). ΔIL1β(%) in
lysates predicted response (p = 0.025, AUC = 0.81; accuracy = 84.6%) outperforming ΔIL1β(%) in plasma (p = 0.77, AUC=0.52) and was robust to adjusting for BMI. In conclusion, ΔIL1β(%) in mononuclear lysates may be a longitudinal biomarker of antidepressant response, potentially helpful in avoiding untimely switching of antidepressants, thereby warranting further investigation.