氧化毁伤是衰老的重要缘由。龙眼多糖的抗氧化和抗衰老活性,特别是纯化的龙眼多糖,还没有被彻底调查。因此,本研究旨在探讨龙眼粗多糖和纯化多糖的抗氧化和抗衰老活性及其作用机制。从龙眼粗多糖LP中分离出纯化的酸性龙眼多糖LP-A。随后,它的结构表征,抗衰老活性及机理研究。结果表明,LP-A为酸性杂多糖,平均分子量(Mw)为4.606×104Da,由9个单糖组成。体外对ABTS自由基的清除率达到99%。在线虫生命实验中,0.3mg/mLLP组和LP-A组能使线虫平均寿命延长9.31%和25.80%,分别。在氧化应激刺激下,LP-A组能使线虫存活时间延长69.57%。在机制方面,龙眼多糖可调节胰岛素/胰岛素样生长因子(IIS)信号通路,增加抗氧化酶的活性,减少脂质过氧化,增强身体对压力损伤的抵抗力,并有效延长线虫的寿命。总之,LP-A比粗多糖LP具有更好的抗衰老活性,它具有作为抗衰老药物的巨大潜力。
Oxidative damage is an important cause of aging. The antioxidant and anti-aging activities of Longan polysaccharides, especially purified Longan polysaccharides, have not been thoroughly investigated. Therefore, this study aimed to investigate the antioxidant and anti-aging activities and mechanisms of crude polysaccharides and purified polysaccharides from Longan. A purified acidic Longan polysaccharide LP-A was separated from Longan crude polysaccharide LP. Subsequently, its structural characterization, anti-aging activity and mechanism were studied. The results showed that LP-A was an acidic heteropolysaccharide with an average molecular weight (Mw) of 4.606 × 104 Da which was composed of nine monosaccharides. The scavenging rate of ABTS free radical in vitro reached 99 %. In the nematode life experiment, 0.3 mg/mL LP group and LP-A group could prolong the average lifespan of nematodes by 9.31 % and 25.80 %, respectively. Under oxidative stress stimulation, LP-A group could prolong the survival time of nematodes by 69.57 %. In terms of mechanism, Longan polysaccharide can regulate insulin / insulin-like growth factor (IIS) signaling pathway, increase the activity of antioxidant enzymes, reduce lipid peroxidation, enhance the body\'s resistance to stress damage, and effectively prolong the lifespan of nematodes. In conclusion, LP-A has better anti-aging activity than crude polysaccharide LP, which has great potential for developing as an anti-aging drug.