嗜热真菌具有多种生物技术和工业应用,例如热稳定酶的生产,生物降解,烟草加工,等。嗜热真菌在低于20°C的温度下无法存活。由于它们无法在低温下生长,他们不稳定,所以放袜是非常困难的。尽管有大量不同的存储方法可用和描述,没有任何方法可以普遍适用于所有真菌。嗜热真菌呈现“嗜热”特征,因此,对其保存提出了新的挑战,并且没有通用的协议来保存嗜热真菌。这项研究的目的是评估生存能力,在不同保存方法下保存的嗜热真菌的污染和稳定性。在这项工作中,25种嗜热霉菌的嗜热真菌分离株,根瘤菌,灰黄三毛,黑心果albomyces,Malbranchea肉桂,嗜热链球菌,Hinnuleus嗜热细菌,Thermothielaioidesterrestris,嗜热支原体,Humicolainsolens在室温下保持恒定的传代培养,+4°C和+20°C,在+4°C冻干,在-20°C冷冻,在-20°C下冷冻块,并在室温下进行5年的新技术液体保存。我们通过在sabouraud葡萄糖琼脂(SDA)或酵母提取物可溶性淀粉琼脂(YpSs)上进行传代培养来评估保存方法对生长的影响,孢子的产生和活力以及宏观和微观形态。在这项研究中,首次对嗜热真菌的保存方法进行了广泛的研究,清楚地表明,在-20°C下冷冻块法和冻干法是长期保存长达5年的更好方法。
Thermophilic fungi have several biotechnological and industrial applications such as thermostable enzyme production, biodegradation, and tobacco processing, etc. Thermophilic fungi cannot survive at temperatures below 20 °C. Owing to their inability to grow at low temperatures, they are not stable, so stocking is very difficult. Although a large number of different storage methods are available and described, no method can be universally applied to all fungi. Thermophilic fungi present \"heat-loving\" characteristics, and therefore a new challenge for its preservation and there is no universal protocol for the preservation of thermophilic fungi. The aim of this study was to evaluate the viability, contamination and stability of thermophilic fungi stored under different preservation methods. In this work, 25 thermophilic fungal isolates of species Thermomyces thermophilus, Rhizomucor pusillus, Trichocladium griseum, Melanocarpus albomyces, Malbranchea cinnamomea, Thermothelomyces thermophilus, Thermothelomyces hinnuleus,Thermothielavioidesterrestris, Mycothermus thermophilus, Humicola insolens maintained constant sub-culturing at room temperature, +4 °C and +20 °C, lyophilization at +4 °C, freezing at -20 °C, freezing block at -20 °C and a new technique liquid preservation at room temperature for the periods ranging 5 years. We evaluated the effect of preservation methods by sub-culturing onto either sabouraud dextrose agar (SDA) or yeast extract soluble starch agar (YpSs) on growth, production and viability of spores and macro- and micromorphology. In this study, preservation methods for thermophilic fungi were investigated extensively for the first time and it is clearly shown that freezing block at -20 °C method and lyophilization were better methods for long-term preservation up to 5 years.