有机氯农药(OCPs)是在全球水生环境中发现的持久性有机化合物。经过充分验证和完善的分析方法对于检测环境中的OCP至关重要。在这项研究中,开发并评估了一种定量水中OCPs的分析方法。这里,线性范围,再现性,不确定性,特异性,方法检测限(MDL),并特别强调检测和定量限进行了评估。进行回收率研究以测量该方法的准确度和精密度。该方法在2.5-20μg/L范围内表现出良好的线性。由于在无基线噪声的空白样品的色谱图中未检测到目标化合物,检测限(LOD)和定量限(LOQ)使用线性回归方法确定,外部校准曲线斜率,和实验室强化的空白检测。所有化合物显示不同的LOD和LOQ值,取决于所使用的方法。特别是,硫丹硫酸盐,甲氧基氯,异狄氏剂酮,H.环氧化物,七氯,和4,4'-DDT表现出较高的检测限。15种化合物在5μg/L加标浓度下的回收率在50~150%之间,这与APHA方法的准确性是一致的。除硫丹硫酸盐外,所有其他化合物的相对标准偏差均低于20%,显示良好的精度。该方法也已应用于实际水样。这种验证技术是可靠的,敏感,简单,快速,容易理解,和可重复的。还对该方法在实际水样中的应用进行了研究。在水样中仅检测到α-BHC和γ-氯丹。
Organochlorine pesticides (OCPs) are persistent organic compounds found in aquatic environments worldwide. A well-validated and well-established analytical method is crucial for detecting OCPs in the environment. In this study, an analytical method for quantifying OCPs in water was developed and evaluated. Here, the range of linearity, reproducibility, uncertainty, specificity, method detection limits (MDL), and special emphasis on detection and quantitation limits were assessed. Recovery studies were performed to measure the accuracy and precision of the method. This method exhibited excellent linearity in the range of 2.5-20 μg/L for all compounds. As none of the targeted compounds was detected in the chromatograms of the blank sample with no baseline noise, the limits of detection (LOD) and limits of quantification (LOQ) were determined using the linear regression method, external calibration curve slope, and laboratory fortified blank-based detection. All compounds showed different LOD and LOQ values, depending on the approach used. In particular, endosulfan sulfate, methoxychlor, endrin ketone, H. epoxide, heptachlor, and 4,4\'-DDT exhibited high detection limits. The recovery percentage of the 15 compounds at 5 μg/L spiked concentration was between 50 and 150 %, which is consistent with the accuracy of the APHA method. Except for endosulfan sulfate, the relative standard deviations of all other compounds were below 20 %, indicating good precision. This method has also been applied to real water samples. This validation technique is reliable, sensitive, simple, rapid, easy to comprehend, and reproducible. The application of this method in the real water samples was also conducted. Only α-BHC and γ-Chlordane were detected in the water sample.