背景：主动脉夹层（AD）是一种严重的疾病。以前的研究,使用外周血生物标志物诊断AD具有很强的临床可行性，但可能的分子机制尚不清楚。
方法：来自79名健康受试者（HC）的血清，73例AD患者，并通过LC-MS检测74例确诊的急性心肌梗死（AMI）患者的代谢物（AFMK，甘油磷酸胆碱，肌苷,SPH)。采用液相芯片技术检测三组细胞因子的表达。
结果：以AMI和AD患者血清4项代谢指标的含量变化趋势作为诊断模型。有效诊断率为97.8%。区分AMI患者和AD患者的诊断率为89.8％。3组血清中23种细胞因子的表达存在显著差异。通过相关性分析，发现miP-1，IL-7，MIP-1β，EGF和其他细胞因子与四种代谢分子显著相关。
结论：AFMK，甘油磷酸胆碱，肌苷,SphingfunginB（SPH）代谢物是AD的潜在生物标志物，相关代谢过程的影响可能与miP-1、IL-7、MIP-1β、EGF等细胞因子。
BACKGROUND: Aortic dissection (AD) is a serious disease. Previous study, the use of peripheral blood biomarkers to diagnose AD showed strong clinical feasibility, but the possible molecular mechanism is unclear.
METHODS: Sera from 79 healthy subjects, 73 patients with well-established AD, and 74 patients with well-established acute myocardial infarction (AMI) were investigated by Liquid Chromatograph-Mass Spectrometer to detect metabolites (AFMK, Glycerophosphocholine, Inosine, SPH). The cell factor expression in the 3 group were detected by Liquid Chip Technology.
RESULTS: The serum content trends of 4 metabolic indexes in patients with AMI and AD group were used as the diagnostic models, and the effective diagnosis rate was 97.8%. The diagnosis rate is 89.8% in distinguishing patients with AMI from patients with AD. The expression in serum of the 3 groups showed that there were significant differences in the expression of 23 cytokines. By correlation analysis, it was found that miP-1, IL-7, MIP-1β, EGF and other cytokines were significantly correlated with the 4 metabolic molecules.
CONCLUSIONS: AFMK, Glycerophosphocholine, Inosine, Sphingfungin B (SPH) metabolites are potential biomarkers for AD, and the influence of related metabolic process may be related to the expression of miP-1, IL-7, MIP-1β, EGF, and other cytokines.
