产油酵母在废水处理中可以在降解苯酚的同时产生脂质。在这项研究中,采用Plackett-Burman(PB)设计,使用Rtoruloides9564T确定苯酚降解和脂质生产的关键因素。而温度,接种物大小,和搅拌对这两个过程都很显著(p<0.05),pH值和培养对脂质生产有重要意义,和苯酚去除,分别。来自四个因素的结果(pH,温度,接种物大小,和潜伏期)使用中央复合旋转设计(CCD)实验来建立二次和遗传算法优化的ANN模型。在训练集上,苯酚降解(R2:0.993)和脂质生产(R2:0.958)的简化二次模型略低于ANN模型(分别为R2:0.999、0.982)。同等重要的多目标优化表明苯酚降解在106.4-108.76%之间,脂质产量为0.864-0.903g/L,通过多项式和人工神经网络模型。在pH6.07,接种物大小14.68%v/v时,在脂质生产中获得了完全的苯酚降解(100%)和3.35倍的增加(0.918g/L),在29.5°C下进行92.17h的实验。
Oleaginous yeast can produce lipids while degrading phenol in wastewater treatment. In this study, a Plackett-Burman Design (PBD) was adopted to identify key factors of phenol degradation and lipid production using R toruloides 9564T. While temperature, inoculum size, and agitation were significant for both the processes (p < 0.05), pH and incubation were significant for lipid production, and phenol removal, respectively. Results from four factors (pH, temperature, inoculum size, and incubation period) central composite design (CCD) experiment were used to formulate quadratic and genetic algorithm-optimized ANN models. The reduced quadratic model for phenol degradation (R2: 0.993) and lipid production (R2: 0.958) were marginally inferior to ANN models (R2: 0.999, 0.982, respectively) on training sets. Multi-objective optimization with equal importance suggests phenol degradation between 106.4 and 108.76%, and lipid production of 0.864-0.903 g/L, by polynomial and ANN models. Complete phenol degradation (100%) and 3.35-fold increment (0.918 g/L) in lipid production were obtained at pH 6.07, inoculum size 14.68% v/v, at 29.5 °C in 92.17 h experimentally.