龋齿目前被认为是口腔生物膜内的生态失衡,导致牙齿的硬组织溶解。传统上认为属于链球菌群的两个物种,变形链球菌(SM)和血链球菌(SS),是龋齿发病的病因。
■本体内研究是对40名龋齿活跃(CA)和无龋齿(CF)的儿童进行的。他们被分成两组,I组(CA)=20,II组(CF)=20。将整个唾液收集到具有缓冲溶液的小瓶中并储存在冷藏中。进行聚合酶链反应(PCR)以鉴定并关联CA和CF儿童的SM和SS。
■CA组和CF组之间的平均SM水平的比较在p=0.001时显示出统计学上的显着结果。龋齿评分与SM之间的Spearman相关性显示龋齿评分与SM之间的0.77强相关性,在p=0.001时具有统计学意义。同样,SS和龋齿评分显示0.22的弱相关性。对SM和龋齿评分的简单线性回归分析显示,龋齿评分增加1分,显著增加4.74个单位,具有统计学意义。
■患有龋齿的儿童中SM水平的存在是显著的,然而,在CF儿童中,SS水平以增加的水平存在。龋齿评分与SM之间存在很强的相关性。简单线性回归分析预测在p<0.001时,每增加1分龋齿4.74个单位的统计学显著增加。随着龋齿的增加,SM计数增加,但SS计数减少;随着SS计数的增加,SM计数减少。
■ThimmegowdaU,贝拉加塔五世,ChikkanarasaiahN,etal.龋齿活跃和无龋齿儿童中变异链球菌和血链球菌的鉴定和相关性:PCR研究。IntJClinPediatrDent2023;16(1):9-15。
UNASSIGNED: Dental caries is currently considered an ecological imbalance within the oral biofilm leading to the dissolution of the tooth\'s hard tissues. It has been traditionally thought that two species belonging to the Streptococci group, Streptococcus mutans (SM) and Streptococcus sanguinis (SS), are the etiologically responsible for the onset of dental decay.
UNASSIGNED: The present in vivo study was conducted on 40 children with caries-active (CA) and caries-free (CF). They were allocated into two groups, group I (CA) = 20 and group II (CF) = 20. The whole saliva was collected into the vials with buffer solution and was stored in cold storage. Polymerase chain reaction (PCR) was done to identify and correlate SM and SS in CA and CF children.
UNASSIGNED: Comparison of mean SM level between CA and CF groups showed a statistically significant result at p = 0.001. Spearman\'s correlation between caries score and SM showed a strong correlation of 0.77 between caries score and SM, which was statistically significant at p = 0.001. Similarly, SS and caries scores showed a weak correlation of 0.22. Simple linear regression analysis to SM and caries score showed a significant increase of 4.74 units for 1 score increase in caries score, which is statistically significant.
UNASSIGNED: The presence of SM levels in children with caries is significant, whereas, in CF children, SS levels are present in increased levels. A strong correlation was seen between caries scores and SM. The simple linear regression analysis predicts a statistically significant increase by 4.74 units per increase of 1 score of caries at p < 0.001. As caries increase, SM count increases, but SS count decreases; as SS count increases, there is a reduction in SM counts.
UNASSIGNED: Thimmegowda U, Belagatta V, Chikkanarasaiah N, et al. Identification and Correlation of Streptococcus mutans and Streptococcus sanguinis in Caries-active and Caries-free Children: A PCR Study. Int J Clin Pediatr Dent 2023;16(1):9-15.