Accurate interpretation of in vivo wide-field fluorescent imaging (WFFI) data requires precise separation of raw fluorescence signals into neural and hemodynamic components. The classical Beer-Lambert law-based approach, which uses concurrent 530-nm illumination to estimate relative changes in cerebral blood volume (CBV), fails to account for the scattering and reflection of 530-nm photons from non-neuronal components leading to biased estimates of CBV changes and subsequent misrepresentation of neural activity. This study introduces a novel linear regression approach designed to overcome this limitation. This correction provides a more reliable representation of CBV changes and neural activity in fluorescence data. Our method is validated across multiple datasets, demonstrating its superiority over the classical approach.

OBJECTIVE: To provide a navigator-based run-time motion and first-order field correction for three-dimensional human brain imaging with high precision, minimal calibration and acquisition, and fast processing.
METHODS: A complex-valued linear perturbation model with feedback control is extended to estimate and correct for gradient shim fields using orbital navigators (2.3 ms). Two approaches for sensitizing the model to gradient fields are presented, one based on finite differences with three additional navigators, and another projection-based approximation requiring no additional navigators. A mechanism for noise decorrelation of the matrix and the data is proposed and evaluated to reduce unwanted parameter biases.
RESULTS: The rigid motion and first-order field control achieves robust motion and gradient shim corrections improving image quality in a series of phantom and in vivo experiments with varying field conditions. In phantom scans, magnet drifts, forced gradient field perturbations and field distortions from shifts of a second bottle phantom are successfully corrected. Field estimates of the magnet drifts are in good agreement with concurrent field probe measurements. For in vivo scans, the proposed method mitigates field variations from torso motions while being robust to head motion. In vivo gradient field precisions were 30   nT / m $$ 30\\;\\mathrm{nT}/\\mathrm{m} $$ along with single-digit micrometer and millidegree rigid precisions.
CONCLUSIONS: The navigator-based method achieves accurate, high-precision run-time motion and field corrections with low sequence impact and calibration requirements.

The Neuroimaging Data Model (NIDM) is a series of specifications for describing all aspects of the neuroimaging data lifecycle from raw data to analyses and provenance. NIDM uses community-driven terminologies along with unambiguous data dictionaries within a Resource Description Framework (RDF) document to describe data and metadata for integration and query. Data from different studies, using locally defined variable names, can be retrieved by linking them to higher-order concepts from established ontologies and terminologies. Through these capabilities, NIDM documents are expected to improve reproducibility and facilitate data discovery and reuse. PyNIDM is a Python toolbox supporting the creation, manipulation, and querying of NIDM documents. Using the query tools available in PyNIDM, users are able interrogate datasets to find studies that have collected variables measuring similar phenotypic properties. This, in turn, facilitates the transformation and combination of data across multiple studies. The focus of this manuscript is the linear regression tool which is a part of the PyNIDM toolbox and works directly on NIDM documents. It provides a high-level statistical analysis that aids researchers in gaining more insight into the data that they are considering combining across studies. This saves researchers valuable time and effort while showing potential relationships between variables. The linear regression tool operates through a command-line interface integrated with the other tools (pynidm linear-regression) and provides the user with the opportunity to specify variables of interest using the rich query techniques available for NIDM documents and then conduct a linear regression with optional contrast and regularization.

OBJECTIVE: Delta bilirubin (albumin-covalently bound bilirubin) may provide important clinical utility in identifying impaired hepatic excretion of conjugated bilirubin, but it cannot be measured in real-time for diagnostic purposes in clinical laboratories.
METHODS: A total of 210 samples were collected, and their delta bilirubin levels were measured four times using high-performance liquid chromatography. Data collected included age, sex, diagnosis code, delta bilirubin, total bilirubin, direct bilirubin, total protein, albumin, globulin, aspartate aminotransferase, alanine transaminase, alkaline phosphatase, gamma-glutamyl transferase, lactate dehydrogenase, hemoglobin, serum hemolysis value, hemolysis index, icterus value (Iv), icterus index (Ii), lipemia value (Lv), and lipemia index. To conduct feature selection and identify the optimal combination of variables, linear regression machine learning was performed 1,000 times.
RESULTS: The selected variables were total bilirubin, direct bilirubin, total protein, albumin, hemoglobin, Iv, Ii, and Lv. The best predictive performance for high delta bilirubin concentrations was achieved with the combination of albumin-direct bilirubin-hemoglobin-Iv-Lv. The final equation composed of these variables was as follows: delta bilirubin = 0.35 × Iv + 0.05 × Lv - 0.23 × direct bilirubin - 0.05 × hemoglobin - 0.04 × albumin + 0.10.
CONCLUSIONS: The equation established in this study is practical and can be easily applied in real-time in clinical laboratories.

Linear Mixed Effects (LME) models are powerful statistical tools that have been employed in many different real-world applications such as retail data analytics, marketing measurement, and medical research. Statistical inference is often conducted via maximum likelihood estimation with Normality assumptions on the random effects. Nevertheless, for many applications in the retail industry, it is often necessary to consider non-Normal distributions on the random effects when considering the unknown parameters\' business interpretations. Motivated by this need, a linear mixed effects model with possibly non-Normal distribution is studied in this research. We propose a general estimating framework based on a saddlepoint approximation (SA) of the probability density function of the dependent variable, which leads to constrained nonlinear optimization problems. The classical LME model with Normality assumption can then be viewed as a special case under the proposed general SA framework. Compared with the existing approach, the proposed method enhances the real-world interpretability of the estimates with satisfactory model fits.

BACKGROUND: COVID-19 protective behaviors are key interventions advised by the World Health Organization (WHO) to prevent COVID-19 transmission. However, achieving compliance with this advice is often challenging, particularly among socially vulnerable groups.
OBJECTIVE: We developed a social vulnerability index (SVI) to predict individuals\' propensity to adhere to the WHO advice on protective behaviors against COVID-19 and identify changes in social vulnerability as Omicron evolved in African countries between January 2022 and August 2022 and Asia Pacific countries between August 2021 and June 2022.
METHODS: In African countries, baseline data were collected from 14 countries (n=15,375) during the first Omicron wave, and follow-up data were collected from 7 countries (n=7179) after the wave. In Asia Pacific countries, baseline data were collected from 14 countries (n=12,866) before the first Omicron wave, and follow-up data were collected from 9 countries (n=8737) after the wave. Countries\' socioeconomic and health profiles were retrieved from relevant databases. To construct the SVI for each of the 4 data sets, variables associated with COVID-19 protective behaviors were included in a factor analysis using polychoric correlation with varimax rotation. Influential factors were adjusted for cardinality, summed, and min-max normalized from 0 to 1 (most to least vulnerable). Scores for compliance with the WHO advice were calculated using individuals\' self-reported protective behaviors against COVID-19. Multiple linear regression analyses were used to assess the associations between the SVI and scores for compliance to WHO advice to validate the index.
RESULTS: In Africa, factors contributing to social vulnerability included literacy and media use, trust in health care workers and government, and country income and infrastructure. In Asia Pacific, social vulnerability was determined by literacy, country income and infrastructure, and population density. The index was associated with compliance with the WHO advice in both time points in African countries but only during the follow-up period in Asia Pacific countries. At baseline, the index values in African countries ranged from 0.00 to 0.31 in 13 countries, with 1 country having an index value of 1.00. The index values in Asia Pacific countries ranged from 0.00 to 0.23 in 12 countries, with 2 countries having index values of 0.79 and 1.00. During the follow-up phase, the index values decreased in 6 of 7 African countries and the 2 most vulnerable Asia Pacific countries. The index values of the least vulnerable countries remained unchanged in both regions.
CONCLUSIONS: In both regions, significant inequalities in social vulnerability to compliance with WHO advice were observed at baseline, and the gaps became larger after the first Omicron wave. Understanding the dimensions that influence social vulnerability to protective behaviors against COVID-19 may underpin targeted interventions to enhance compliance with WHO recommendations and mitigate the impact of future pandemics among vulnerable groups.

To improve prediction performance and reduce artifacts in Raman spectra, we developed an eXtreme Gradient Boosting (XGBoost) preprocessing method to preprocess the Raman spectra of glucose, glycerol and ethanol mixtures. To ensure the robustness and reliability of the XGBoost preprocessing method, an X-LR model (which combined XGBoost preprocessing and a linear regression (LR) model) and a X-MLP model (which combined XGBoost preprocessing and a multilayer perceptron (MLP) model) were developed. These two models were used to quantitatively analyze the concentrations of glucose, glycerol and ethanol in the Raman spectra of mixed solutions. The proportion map of hyperparameters was firstly used to narrow down the search range of hyperparameters in the X-LR and the X-MLP models. Then the correlation coefficients (R2), root mean square of calibration (RMSEC), and root mean square error of prediction (RMSEP) were used to evaluate the models\' performance. Experimental results indicated that the XGBoost preprocessing method achieved higher accuracy and generalization capability, with better performance than those of other preprocessing methods for both LR and MLP models.

Vegetable quality parameters are established according to standards primarily based on visual characteristics. Although knowledge of biochemical changes in the secondary metabolism of plants throughout development is essential to guide decision-making about consumption, harvesting and processing, these determinations involve the use of reagents, specific equipment and sophisticated techniques, making them slow and costly. However, when non-destructive methods are employed to predict such determinations, a greater number of samples can be tested with adequate precision. Therefore, the aim of this work was to establish an association capable of modeling between non-destructive-physical and colorimetric aspects (predictive variables)-and destructive determinations-bioactive compounds and antioxidant activity (variables to be predicted), quantified spectrophotometrically and by HPLC in \'Nanicão\' bananas during ripening. It was verified that to predict some parameters such as flavonoids, a regression equation using predictive parameters indicated the importance of R2, which varied from 83.43 to 98.25%, showing that some non-destructive parameters can be highly efficient as predictors.

OBJECTIVE: Midwives are susceptible to burnout due to the physically and emotionally demanding nature of their job. Burnout is an occupational phenomenon with far-reaching consequences.
OBJECTIVE: This study aimed to assess the magnitude of burnout and predictors among midwives working at public hospitals in northwest Ethiopia.
METHODS: An institutional-based cross-sectional study was conducted from February 7 to April 30, 2022. A simple random sampling method was employed to include 640 study participants. Data were collected using a self-administered questionnaire, entered into Epi-data 4.6 software, and exported to SPSS version 25 for analysis. A multivariable linear regression analysis model was fitted to identify factors contributing to midwives\' burnout.
RESULTS: The overall prevalence of midwives\' burnout was 55.3 % (95 % CI = 51.7-58.9). The prevalence of personal, work-related, and client-related burnout was 58.3 %, 60.3 %, and 55.5 %, respectively. Factors that were significantly associated with burnout includes workplace violence (β = 5.02, CI: 2.90, 7.13), not receiving training (β = 4.32 CI: 1.81, 6.80), being exposed to blood and body fluids or needle stick injuries (β = 5.13 CI: 3.12, 7.13), low superior support (β = 5.13 CI: 1.94, 5.30), working in tertiary hospitals (β = 12.77 CI: 9.48, 16.06), and job rotation of six months or less (β = 16.75, CI: 13.12, 20.39).
CONCLUSIONS: This study found that the prevalence of burnout among midwives was significantly high. Addressing burnout requires implementing effective burnout prevention measures including enhancing management support, offering professional training, creating a conducive working environment, and adhering to standard precautions.

Heart failure (HF) is defined as the inability of the heart to meet body oxygen demand requiring an elevation in left ventricular filling pressures (LVP) to compensate. LVP increase can be assessed in the cardiac catheterization laboratory, but this procedure is invasive and time-consuming to the extent that physicians rather rely on non-invasive diagnostic tools. In this work, we assess the feasibility to develop a novel machine-learning (ML) approach to predict clinically relevant LVP indices. Synchronized invasive (pressure-volume tracings) and non-invasive signals (ECG, pulse oximetry, and cardiac sounds) were collected from anesthetized, closed-chest Göttingen minipigs. Animals were either healthy or had HF with reduced ejection fraction and circa 500 heartbeats were included in the analysis for each animal. The ML algorithm showed excellent prediction of LVP indices estimating, for instance, the end-diastolic pressure with a R2 of 0.955. This novel ML algorithm could assist clinicians in the care of HF patients.