糖转运蛋白在植物生长发育中发挥重要作用,开花和结果,以及对不利的非生物和生物环境条件的反应。百合(百合。)是一些最具代表性的观赏性球茎花。糖代谢对于百合鳞茎的形成至关重要;因此,阐明糖转运蛋白的数量和表达模式对于进一步分析其在鳞茎形成中的作用至关重要。在这项研究中,根据东方百合“Sorbonne”和百合×formolongi的转录组数据,在“Sorbonne”和百合×formolongi中总共鉴定出69和41种糖转运蛋白,分别,通过进行生物信息学分析。通过系统发育分析,单糖转运蛋白(MST)可分为七个亚家族,蔗糖转运蛋白(SUT)可分为三个亚组,和糖最终将出口运输(SWEET)可分为四个分支。根据对保守图案的分析,在MST中预测了20、14和12个保守基序,SUTs,和瑞典,分别。保守域分析表明,MST和SUT包含单个域,而大多数SWEET都有两个MtN3/唾液结构域,也称为PQ环重复。LohINT1被预测具有较少数量的跨膜结构结构域,克隆并分析亚细胞定位。发现LohINT1蛋白主要位于细胞膜中。此外,表达分析表明,22个LohMSTs,1个LohSUT,和5个LohSWEETs在\'Sorbonne\'1天后上调,这表明它们可以调节小丸的启动。总共10个LflMSTs,1个LflSUTs,播种后4~6个月,6个LflSWEET上调,对应于百合×formolongi的青少年到成人的过渡阶段,这表明它们也可能在伴随的灯泡肿胀过程中发挥作用。结合实时定量PCR(qRT-PCR)分析,LohSTP8和LohSTP12在小球启动的极早期阶段显着过表达,LflERD6.3在地下鳞茎生长过程中显著过度表达,表明它们可能是百合鳞茎形成的关键糖转运蛋白,这需要进一步的功能验证。
Sugar transporters play important roles in plant growth and development, flowering and fruiting, as well as responses to adverse abiotic and biotic environmental conditions. Lilies (
Lilium spp.) are some of the most representative ornamental bulbous flowers. Sugar metabolism is critical for bulb formation in lilies; therefore, clarifying the amount and expression pattern of sugar transporters is essential for further analyzing their roles in bulb formation. In this study, based on the transcriptome data of the
Lilium Oriental hybrid \'Sorbonne\' and
Lilium × formolongi, a total of 69 and 41 sugar transporters were identified in \'Sorbonne\' and
Lilium × formolongi, respectively, by performing bioinformatics analysis. Through phylogenetic analysis, monosaccharide transporters (MSTs) can be divided into seven subfamilies, sucrose transporters (SUTs) can be divided into three subgroups, and sugars will eventually be exported transporters (SWEETs) can be divided into four clades. According to an analysis of conserved motifs, 20, 14, and 12 conserved motifs were predicted in MSTs, SUTs, and SWEETs, respectively. A conserved domain analysis showed that MSTs and SUTs contained a single domain, whereas most of the SWEETs harbored two MtN3/saliva domains, also known as a PQ-loop repeat. The LohINT1, which was predicted to have a smaller number of transmembrane structural domains, was cloned and analyzed for subcellular localization. It was found that the LohINT1 protein is mainly localized in the cell membrane. In addition, the expression analysis indicated that 22 LohMSTs, 1 LohSUTs, and 5 LohSWEETs were upregulated in \'Sorbonne\' 1 day after scale detachment treatment, suggesting that they may regulate the initiation of the bulblet. A total of 10 LflMSTs, 1 LflSUTs, and 6 LflSWEETs were upregulated 4~6 months after sowing, which corresponds to the juvenile-to-adult transition phase of
Lilium × formolongi, suggesting that they may also play a role in the accompanying bulb swelling process. Combined with quantitative real-time PCR (qRT-PCR) analysis, LohSTP8 and LohSTP12 were significantly overexpressed during the extremely early stage of bulblet initiation, and LflERD6.3 was significantly overexpressed during the growth of the underground bulblet, suggesting that they may be key sugar transporters in the formation of lily bulbs, which needs further functional verification.