特应性皮炎(AD)是一种慢性皮肤疾病,其特征是严重的湿疹炎症,肿胀,和苔藓化。在AD的慢性期,过敏原对T辅助(Th)-22细胞的激活会导致表皮增生和角化过度。Derma-Hc由五种具有抗AD作用的天然草药组成,比如黄芪,荆棘。,阴囊。,当归,在这项研究中,研究了Derma-Hc对2,4-二硝基氯苯(DNCB)诱导的AD皮肤苔藓化的改善作用。小鼠的背部皮肤用DNCB致敏以诱导AD样皮肤损伤。评估皮炎评分和刮伤频率。用H&E染色测量表皮和真皮的厚度。此外,用甲苯胺蓝染色观察到肥大细胞的浸润。然后,桥粒钙粘蛋白,通过免疫荧光检查DSC1。通过RT-PCR和Western印迹分析了在AD样皮肤病变和TNF-αIFN-γ处理的人角质形成细胞包括角质形成细胞分化基因和IL-22的JAK1-STAT3信号通路中参与苔藓化的病理机制。Derma-Hc的局部治疗可改善AD样症状,如干燥,水肿和苔藓化,并减少划痕的数量。组织病理学分析显示Derma-Hc显著抑制表皮增生,角化过度,肥大细胞浸润.此外,DSC1水平在表皮中通过Derma-Hc高表达。此外,FLGmRNA表达水平,表皮分化复合物基因,通过Derma-Hc治疗恢复。KLK5和KLK7明显减少,使背侧皮肤组织和人角质形成细胞的角质形成细胞分化正常化。另一方面,Derma-Hc恢复SPINK5的表达水平。此外,Derma-Hc通过阻断JAK1-STAT3信号通路抑制IL-22。一起来看,Derma-Hc,天然草药配方,调节角质形成细胞分化并抑制角化过度的表皮增生。因此,Derma-Hc可能是通过调节IL-22相关皮肤苔藓化的信号传导来治疗慢性AD的有希望的候选者。
Atopic dermatitis (AD) is a chronic cutaneous disorder that is characterized by severe eczematous inflammation, swelling, and
lichenification. Activation of T helper (Th)-22 cells by allergens leads to epidermal hyperplasia with hyperkeratosis at the chronic phase of AD. Derma-Hc is composed of five natural herbs with anti-AD effects, such as Astragalus membranaceus BUNGE, Schizonepeta tenuifolia Briq., Cryptotympana pustulata Fabr., Angelica sinensis Diels, Arctium lappa L. In this study, the ameliorative effect of Derma-Hc on cutaneous
lichenification in 2,4-dinitrochlorobenzne (DNCB)-induced AD was investigated. The dorsal skin of mice was sensitized with DNCB to induce AD-like skin lesions. The dermatitis score and frequency of scratching were evaluated. Thickness of epidermis and dermis was measured by staining with H&E. In addition, infiltration of the mast cell was observed by staining with toluidine blue. Then, desmosomal cadherin, DSC1 was examined by immunofluorescence. Pathological mechanisms involved in
lichenification were analyzed in AD-like skin lesions and TNF-α + IFN-γ-treated with human keratinocytes including keratinocyte differentiation genes and JAK1-STAT3 signaling pathway with IL-22 by RT-PCR and western blotting. Topical treatment of Derma-Hc improved AD-like symptoms such as dryness, edema and lichenefication and decreased the number of scratches. Histopathological analysis demonstrated that Derma-Hc significantly inhibited epidermal hyperplasia, hyperkeratosis, and mast cells infiltration. In addition, the level of DSC1 was highly expressed in the epidermis by Derma-Hc. Moreover, mRNA expression level of FLG, an epidermal differentiation complex gene, was recovered by Derma-Hc treatment. KLK5 and KLK7 were markedly reduced to normalize keratinocyte differentiation in dorsal skin tissues and human keratinocytes. On the other hand, Derma-Hc restored expression level of SPINK5. In addition, Derma-Hc inhibited IL-22 via the blockade of JAK1-STAT3 signal pathway. Taken together, Derma-Hc, a natural herbal formula, regulated keratinocyte differentiation and inhibited epidermal hyperplasia with hyperkeratosis. Therefore, Derma-Hc could be a promising candidate for treating chronic AD through modulating signaling of IL-22-associated skin
lichenification.