Shiner进化枝的北美小鱼,在灭虫科中,由于大量的分类单元以及保守的形态,因此代表了Cypriniformes的分类最复杂的进化枝之一。该进化枝中的物种在属和亚属之间移动,直到社区决定将许多形态相似的未分类分类类群归为一个属,Notropis,已保存多达325种。尽管系统发育研究开始重新提升合并为Notropis的某些属,比如Cyprinella,Luxilus,Lythrurus,和翼龙鱼,Notropis大属仍然是许多位置不确定的照者的分类库。测序技术的最新分子进展提供了使用系统基因组标记重新检查Shiner进化枝的机会。使用鱼探针套件,我们对87个物种中的90个标本进行了测序,这些物种代表了Shiner分支中的16个属,得到1,004个基因座和286,455个碱基对的数据集。尽管数据集很大,只有32,349bp(11.29%)具有系统发育信息。在我们的最大似然树中,78%的节点得到100%的自举支持,证明了系统基因组标记在较低分类水平上的实用性。毫不奇怪,Notropis和Hudsonius内的物种,Luxilus,和Alburnops没有被解析为单系组。如果排除callistia,则鲤鱼是单系的,如果包括Hudsoniuscummingsae,则蝶呤是单系的。我们建议的分类学变化是:限制Alburnops和Notropis中包含的物种,水滴虫亚属的海拔,包括在Miniellus中的物种扩张,Hudsoniuscummingsae到翼龙的运动,和球菌属的复活。我们还有三个物种的两个标本,Notropisatherinoides,Ericymbaamplamala,和Pimephales警惕,并发现了地区之间的显着差异(分别为1,086、1,424和845个核苷酸)。
North American minnows of the Shiner Clade, within the family
Leuciscidae, represent one of the most taxonomically complex clades of the order Cypriniformes due to the large number of taxa coupled with conserved morphologies. Species within this clade were moved between genera and subgenera until the community decided to lump many of the unclassified taxa with similar morphologies into one genus, Notropis, which has held up to 325 species. Despite phylogentic studies that began to re-elevate some genera merged into Notropis, such as Cyprinella, Luxilus, Lythrurus, and Pteronotropis, the large genus Notropis remained as a taxonomic repository for many shiners of uncertain placement. Recent molecular advances in sequencing technologies have provided the opportunity to re-examine the Shiner Clade using phylogenomic markers. Using a fish probe kit, we sequenced 90 specimens in 87 species representing 16 genera included in the Shiner Clade, with a resulting dataset of 1,004 loci and 286,455 base pairs. Despite the large dataset, only 32,349 bp (11.29%) were phylogenetically informative. In our maximum likelihood tree, 78% of nodes are 100% bootstrap supported demonstrating the utility of the phylogenomic markers at lower taxonomic levels. Unsurprisingly, species within Notropis as well as Hudsonius, Luxilus, and Alburnops are not resolved as monophyletic groups. Cyprinella is monophyletic if Cyprinella callistia is excluded, and Pteronotropis is monophyletic if it includes Hudsonius cummingsae. Taxonomic changes we propose are: restriction of species included in Alburnops and Notropis, elevation of the subgenus Hydrophlox, expansion of species included in Miniellus, movement of Hudsonius cummingsae to Pteronotropis, and resurrection of the genera Coccotis and Paranotropis. We additionally had two specimens of three species, Notropis atherinoides, Ericymba amplamala, and Pimephales vigilax and found signficant differences between the localities (1,086, 1,424, and 845 nucleotides respectively).