确定体液中临床上显著量的tau蛋白是阿尔茨海默病(AD)诊断中的主要问题。因此,目前的工作旨在开发一种简单的,无标签,快,高度敏感,和选择性2D碳主链氧化石墨烯(GO)图案化的表面等离子体共振(SPR)介导的亲和力生物传感器用于Tau-441监测。最初,非等离子体纳米尺寸的GO是使用改良的悍马方法制成的,而绿色合成的金纳米粒子(AuNP)采用阴离子和阳离子聚电解质进行逐层(LbL)设计。进行了几次光谱评估,以确保GO的合成,AuNPs,和LBL组装。在此之后,使用碳二亚胺化学将抗Tau兔抗体固定在设计的LbL组件上,和各种研究,如敏感性,选择性,稳定性,重复性,加标样品分析,等。,使用构建的亲和GO@LbL-AuNP-Anti-TauSPR生物传感器进行。作为输出,它显示出广泛的浓度范围和非常低的检测限150ng/mL至5fg/mL和13.25fg/mL,分别。该SPR生物传感器的显著灵敏度代表了等离子体激元AuNP和非等离子体激元GO的组合的优点。在存在干扰分子的情况下,它还对Tau-441表现出很大的选择性,这可能是由于将Anti-Tau兔固定在LbL组件的表面上。此外,它确保了高稳定性和可重复性,加标样品分析和AD诱导的动物样品分析证实了GO@LbL-AuNPs-Anti-TauSPR生物传感器用于Tau-441检测的实用性。总之,制造敏感,选择性,稳定,无标签,快,简单,微创GO@LbL-AuNPs-Anti-TauSPR生物传感器将为未来的AD诊断提供替代方案。
The determination of clinically significant amounts of tau protein in bodily fluids is a major problem in Alzheimer\'s disease (AD) diagnosis. As a result, the present work aims to develop a simple, label-free, fast, highly sensitive, and selective 2D carbon backbone graphene oxide (GO) patterned surface plasmon resonance (SPR) mediated affinity biosensor for Tau-441 monitoring. Initially, non-plasmonic nanosized GO was made using a modified Hummers\' method, whereas green synthesized gold nanoparticles (AuNPs) were subjected to a layer-by-layer (LbL) design employing anionic and cationic polyelectrolytes. Several spectroscopical evaluations were carried out to ensure the synthesis of GO, AuNPs, and LbL assembly. Following that, the Anti-Tau rabbit antibody was immobilized on the designed LbL assembly using carbodiimide chemistry, and various studies such as sensitivity, selectivity, stability, repeatability, spiked sample analysis, etc., were conducted using the constructed affinity GO@LbL-AuNPs-Anti-Tau SPR biosensor. As an output, it shows a broad concentration range and a very low detection limit of 150 ng/mL to 5 fg/mL and 13.25 fg/mL, respectively. The remarkable sensitivity of this SPR biosensor represents the merits of a combination of plasmonic AuNPs and a non-plasmonic GO. It also exhibits great selectivity for Tau-441 in the presence of interfering molecules, which may be because of the immobilization of the Anti-Tau rabbit on the surface of the LbL assembly. Furthermore, it ensured high stability and repeatability, while spiked sample analysis and AD-induced animal samples analysis confirmed the practicability of GO@LbL-AuNPs-Anti-Tau SPR biosensor for Tau-441 detection. In conclusion, fabricated sensitive, selective, stable, label-free, quick, simple, and minimally invasive GO@LbL-AuNPs-Anti-Tau SPR biosensor will provide an alternative for AD diagnosis in the future.