发酵乳杆菌能发挥抗衰老作用,但是它们的作用是特定于菌株的,对某些菌株的分子机制知之甚少。本研究调查了从中国发酵泡菜中分离的发酵乳杆菌WC2020(WC2020)的抗衰老作用及其在秀丽隐杆线虫中的作用机制。WC2020将L1期和L4期蠕虫的平均寿命提高了22.67%和12.42%,分别,与大肠杆菌OP50(OP50)相比,秀丽隐杆线虫的标准食物来源.WC2020诱导的寿命伴随着体长和线粒体跨膜电位的增加以及脂质积累的减少以及活性氧和丙二醛的产生。此外,WC2020增加了谷胱甘肽的产量,超氧化物歧化酶,和过氧化氢酶,并改变了许多表型相关基因的转录本。此外,WC2020喂养的jnk-1而不是akt-2或pmk-1功能丧失突变体的寿命与OP50喂养的蠕虫相似。相应地,WC2020显著上调了jnk-1的表达,而不是胰岛素样基因,p38MAPK,bate-catenin,或TGF-β途径。此外,身体长度的增加,线粒体跨膜电位,在jnk-1突变体中未观察到WC2020诱导的抗氧化能力和脂质积累的减少。此外,WC2020增加了daf-16的表达和daf-16::GFP在细胞核中的比例,在WC2020喂养的daf-16功能丧失突变体中,寿命的增加消失了。总之,WC2020激活JNK/DAF-16通路以改善线粒体功能,减少氧化应激,然后延长线虫的寿命,这表明WC2020可能是一种潜在的益生菌,靶向JNK介导的抗氧化途径,用于食品补充剂和生物加工中的抗衰老作用。实际应用:老龄化对全球经济和人类健康具有深远的影响,并且可能因特定的饮食和营养资源而延迟。这项研究表明,发酵乳杆菌WC2020可能是一种潜在的益生菌菌株,用于食品中,通过JNK介导的抗氧化途径促进长寿和健康。
Lactobacillus fermentum can exert antiaging effects, but their roles are strain-specific, and little is known about the molecular mechanisms in some strains. This study investigated the antiaging effects of L. fermentum WC2020 (WC2020) isolated from Chinese fermented pickles and the underlying mechanism of the action in Caenorhabditis elegans. WC2020 enhanced the mean lifespan of L1-stage and L4-stage worms by 22.67% and 12.42%, respectively, compared with Escherichia coli OP50 (OP50), a standard food source for C. elegans. WC2020-induced longevity was accompanied by an increase in body length and mitochondrial transmembrane potential and a reduction in lipid accumulation and the production of reactive oxygen species and malondialdehyde. Moreover, WC2020 increased the production of glutathione, superoxide dismutases, and catalases and altered the transcripts of many phenotype-related genes. Furthermore, WC2020-fed jnk-1 rather than akt-2 or pmk-1 loss-of-function mutants showed similar lifespans to OP50-fed worms. Correspondingly, WC2020 significantly upregulated the expression of jnk-1 rather than genes involved in insulin-like, p38 MAPK, bate-catenin, or TGF-beta pathway. Moreover, the increase in body length, mitochondrial transmembrane potential, and antioxidant capability and the decrease in lipid accumulation induced by WC2020 were not observed in jnk-1 mutants. Additionally, WC2020 increased the expression of daf-16 and the proportion of daf-16::GFP in the nucleus, and increased lifespan disappeared in WC2020-fed daf-16 loss-of-function mutants. In conclusion, WC2020 activated the JNK/DAF-16 pathway to improve mitochondria function, reduce oxidative stress, and then extend the longevity of nematodes, suggesting WC2020 could be a potential probiotic targeting JNK-mediated antioxidant pathway for antiaging in food supplements and bioprocessing. PRACTICAL APPLICATION: Aging has a profound impact on the global economy and human health and could be delayed by specific diets and nutrient resources. This study demonstrated that Lactobacillus fermentum WC2020 could be a potential probiotic strain used in food to promote longevity and health via the JNK-mediated antioxidant pathway.