■令人信服的证据表明,长链非编码RNA(lncRNA)的异常表达与胃癌(GC)发展之间存在很强的关联。尽管如此,差异表达lncRNAs(DElncRNAs)对GC的生物学影响未被仔细研究.
■生物信息学方法用于差异表达分析和靶基因预测。MTT,菌落形成,和Transwell方法用于GC细胞增殖,迁移,和入侵评估。实施蛋白质印迹以测试蛋白质水平。用双荧光素酶和RNA结合蛋白免疫沉淀(RIP)方法测试基因的结合。
■在GC组织和细胞中观察到显著高水平的LINC00460。LINC00460沉默受限增殖,迁移,和GC细胞的侵袭。FISH和核-细胞质分离测定证实了LINC00460在细胞质中的主要存在。生物信息学预测LINC00460与miRNA-143-5p有结合位点,在GC中上调。双荧光素酶和RIP实验也证实了结合关系。LINC00460s和miRNA-133-5p的同时沉默挽救了sh-LINC004600对GC细胞增殖的抑制作用,迁移,和入侵。HMGA2被预测为miRNA-143-5p下游的靶基因,通过双荧光素酶试验验证了它们的结合关系.沉默HMGA2限制GC细胞增殖,入侵,和移民。LINC00460通过结合miRNA-143-5p调节HMGA2表达,从而影响扩散,入侵,和GC细胞的迁移。
■这些发现验证了LINC00460可以通过海绵miRNA-143-5p调节HMGA2,以促进GC增殖,入侵,和移民,这为lncRNAs在GC的发展提供了更深入的理解。
UNASSIGNED: Compelling evidence has manifested a strong association between aberrant expression of long noncoding RNAs (lncRNAs) and gastric carcinoma (GC) development. Nonetheless, biological impacts of differentially expressed lncRNAs (DElncRNAs) on GC are not scrutinized.
UNASSIGNED: Bioinformatics methods were employed for differential expression analysis and target gene prediction. MTT, colony formation, and Transwell methods were implemented for GC cell proliferation, migration, and invasion assessment. Western blot was implemented to test the protein level. The binding of genes was tested with dual-luciferase and RNA binding protein immunoprecipitation (RIP) approaches.
UNASSIGNED: Noticeably high level of
LINC00460 was observed in GC tissues and cells.
LINC00460 silencing constrained proliferation, migration, and invasion of GC cells. FISH and nuclear-cytoplasmic separation assays confirmed the main presentation of LINC00460 in the cytoplasm. Bioinformatics predicted that
LINC00460 had binding sites to miRNA-143-5p, which was upregulated in GC. Dual luciferase and RIP experiments also confirmed the binding relationship. Concurrent silencing of LINC00460s and miRNA-133-5p rescued the repressive influence of sh-LINC004600 on GC cell proliferation, migration, and invasion. HMGA2 was predicted to be a target gene downstream of miRNA-143-5p, their binding relationship was validated via dual luciferase assays. Silencing HMGA2 constrained GC cell proliferation, invasion, and migration.
LINC00460 modulated HMGA2 expression via binding miRNA-143-5p, thereby affecting proliferation, invasion, and migration of GC cells.
UNASSIGNED: These findings validated that
LINC00460 could regulate HMGA2 via sponging miRNA-143-5p to facilitate GC proliferation, invasion, and migration, which provides a deeper understanding of lncRNAs in the development of GC.