背景:冠状名称来源于它们的冠状刺突蛋白与细胞受体结合。它属于冠状病毒科和nideovirales目,包膜病毒,大小范围为65-125纳米,阳性单标准RNA在26.4和31.7kb之间,含有7096个氨基酸。已经检测到四种亚型,它们是alpha,beta,gamma和delta.
方法:从木尔坦地区收集267例covid-19血液和鼻咽样本。从鼻咽样品中提取RNA并运行PCR。用于临床试验的血样,乳酸脱氢酶,血清铁蛋白水平,D-二聚体,TG,胆固醇,thyphoidot,HDL,淋巴细胞计数和CRP。
结果:267例患者中有127例(47.21%)covid-19PCR阳性,显示ORF1ab扩增,E,和N基因,而140名个体为covid-19PCR阴性,未显示ORF1ab扩增,E和N基因。Covid-19PCR阴性的患者,其他分析测试,如乳酸脱氢酶,HDL,铁蛋白,ESR,CBP,D-二聚体,Tg,胆固醇,CRP和CT扫描。受covid-19影响的患者具有较高的D-二聚体值,ESR,中性粒细胞,LDH,CRP和铁蛋白水平高于正常范围。然而,HDL的值,胆固醇和淋巴细胞从正常范围下降。
BACKGROUND: The corona name derived from their crown like spike proteins attach with cell receptors. It belongs to coronaviradae family and nideovirales order, envelop virus, size range 65-125 nm and positive single standard RNA between 26.4 and 31.7 kb and contain 7096 amino acid. There are four subtypes that have been detected these are alpha, beta, gamma and delta.
METHODS: The 267 covid-19 blood and nasopharyngeal samples were collected from Multan region. RNA extraction from nasopharyngeal samples and run the PCR. The blood samples use for clinical tests, Lactate dehydrogenase, serum ferritin level, D-Dimer, TG, cholesterol, thyphoidot, HDL, lymphocyte count and CRP.
RESULTS: 127 (47.21%) out of 267 patients were covid-19 PCR positive and showed the amplification of ORF1ab, E, and N gene, while 140 individuals were covid-19 PCR negative and not showed the amplification of ORF1ab, E and N gene. The patients with negative Covid-19 PCR, the other analysis tests such as lactate dehydrogenase, HDL, ferritin, ESR, CBP, D-Dimer, Tg, cholesterol, CRP and CT scan. The patients effected covid-19 have higher values of D-Dimer, ESR, Neutrophils, LDH, CRP and ferritin level than normal ranges. However, the values of HDL, cholesterol and lymphocytes were decreased from the normal range.