目的:观察自愿性轮跑4周对慢性应激性抑郁模型大鼠抑郁样行为的影响,探讨运动的抗抑郁机制。
方法:在这项观察性研究中,36只Sprague-Dawley大鼠随机分为对照组,应力模型,和应激运动组(12只/组)。对照组不进行干预,应激模型和应激运动组大鼠进行慢性轻度不可预知应激和隔离。应激练习组年夜鼠也行4周自愿轮跑。检测行为改变及海马蛋白和mRNA表达水平。
结果:自愿车轮行驶显着增加了水平和垂直运动,蔗糖摄入量,以及抑郁模型大鼠强迫游泳试验中蔗糖偏好百分比和减少的不动时间。海马肿瘤坏死因子-α,白细胞介素(IL)-6,IL-1β,和喹啉酸水平显著下降,而IL-4,IL-10和犬尿氨酸水平显着升高。犬尿氨酸-3-单加氧酶和3-羟基邻氨基苯甲酸-3,4-双加氧酶mRNA水平下调,犬尿氨酸转氨酶mRNA上调。
结论:自愿性轮跑可改善抑郁模型大鼠的抑郁样行为。其机制可能与犬尿氨酸途径代谢物水平失衡有关,具有神经毒性和神经保护作用,由长期自愿车轮运行引起的。
OBJECTIVE: To observe the effects of 4 weeks of voluntary wheel running on depressive-like behavior in a rat chronic stress-induced depression model to explore the anti-depressive mechanism of exercise.
METHODS: In this observational study, 36 Sprague-Dawley rats were randomly divided into control, stress model, and stress exercise groups (12 rats/group). The control group received no intervention, and the stress model and stress exercise group rats underwent chronic mild unpredictable stress and isolation. The stress exercise group rats also underwent 4 weeks of voluntary wheel running. Behavioral changes and hippocampal protein and mRNA expression levels were detected.
RESULTS: Voluntary wheel running significantly increased horizontal and vertical movements, sucrose intake, and the sucrose preference percentage and reduced immobility time in the forced swimming test in depression model rats. The hippocampal tumor necrosis factor-α, interleukin (IL)-6, IL-1β, and quinolinic acid levels were significantly decreased, while the IL-4, IL-10, and kynurenic acid levels were significantly increased. Kynurenine-3-monooxygenase and 3-hydroxyanthranilate-3, 4-dioxygenase mRNA levels were downregulated, and kynurenine aminotransferase mRNA was upregulated.
CONCLUSIONS: Voluntary wheel running improved depressive-like behavior in depression model rats. The mechanism may be related to a kynurenine pathway metabolite level imbalance, which has neurotoxic and neuroprotective effects, caused by long-term voluntary wheel running.