脑突触核蛋白的功能异常与帕金森病的发病机制有关。突触核蛋白调节各种突触前过程的能力表明它们对不同脑结构记录的脑电图(EEG)的修饰作用。它们之间相互关系的干扰对于神经退行性疾病的发生和发展至关重要。最近,我们已经证明,在缺乏几种突触核蛋白的小鼠中,来自不同脑结构的脑电图频谱之间的差异与突触核蛋白组合的特异性相关。鉴于EEG频谱是结构间关系的间接特征,在这项研究中,我们分析了从不同结构记录的EEG的瞬时值的相干性,作为它们之间"功能连通性"的直接度量.
方法:来自七组敲除(KO)小鼠的脑电图数据,beta,和γ突触核蛋白与一组野生型(WT)小鼠进行了比较。估计皮层(MC)之间的脑电图相干性,壳核(Pt),腹侧被盖区(VTA),和黑质(SN)的所有组合。
结果:脑电相干抑制,主要在β频段,在KO小鼠与WT同窝动物中观察到。在所有KO组中的MC-Pt和VTA-SN相互关系以及在缺乏所有突触核蛋白或仅β突触核蛋白的小鼠中的所有结构间关系中,抑制都是最小的。在其他突触核蛋白缺失的组合中,在KO小鼠中,显着的EEG相干抑制与VTA和SN有关。
结论:根据不同类型的突触核蛋白的不平衡,突触核蛋白的缺失导致脑内EEG相干性的显着衰减。
The malfunctioning of the brain synucleins is associated with pathogenesis of Parkinson\'s disease. Synucleins\' ability to modulate various pre-synaptic processes suggests their modifying effects on the electroencephalogram (EEG) recorded from different brain structures. Disturbances in interrelations between them are critical for the onset and evolution of neurodegenerative diseases. Recently, we have shown that, in mice lacking several synucleins, differences between the frequency spectra of EEG from different brain structures are correlated with specificity of synucleins\' combinations. Given that EEG spectra are indirect characteristics of inter-structural relations, in this study, we analyzed a coherence of instantaneous values for EEGs recorded from different structures as a direct measure of \"functional connectivity\" between them.
METHODS: EEG data from seven groups of knock-out (KO) mice with combined deletions of alpha, beta, and gamma synucleins versus a group of wild-type (WT) mice were compared. EEG coherence was estimated between the cortex (MC), putamen (Pt), ventral tegmental area (VTA), and substantia nigra (SN) in all combinations.
RESULTS: EEG coherence suppression, predominantly in the beta frequency band, was observed in KO mice versus WT littermates. The suppression was minimal in MC-Pt and VTA-SN interrelations in all KO groups and in all inter-structural relations in mice lacking either all synucleins or only beta synuclein. In other combinations of deleted synucleins, significant EEG coherence suppression in KO mice was dominant in relations with VTA and SN.
CONCLUSIONS: Deletions of the synucleins produced significant attenuation of intra-cerebral EEG coherence depending on the imbalance of different types of synucleins.