■氧化克雷伯菌(K.oxytoca)是继肺炎克雷伯菌之后的普通人群中医院感染的第二种细菌原因。这项研究调查了在接受不同恶性肿瘤转诊医院的一组儿童中,产毒素的K.oxytoca菌株的频率及其对抗生素的敏感性。
■入住Mofid儿童医院癌症化疗组的儿童粪便样本,德黑兰,使用常规生化测试和针对pehX基因的聚合酶链反应对伊朗进行了分析,以鉴定K.oxytoca。使用Kirby-Bauer圆盘扩散技术确定了分离的K.oxytoca对治疗该设施感染的常用处方抗生素的抗生素敏感性。此外,通过PCR分析鉴定了K.oxytoca中编码毒素的基因的普遍性。
■对280名参与者的粪便样本进行了研究,其中38个样本[(55.3%(21/38)42名男性和44.7%(17/38)女性)]对各种克雷伯菌属呈阳性。出了这个,使用培养物和常规生化测试,在2.5%(7/280)的粪便中鉴定了催产钾。此外,2.9%(8/280)的参与者的粪便使用PCR分析检测为K.Oxytoca阳性.使用PCR,(2/7)的氧化钾分离株对npsA和npsB基因测试为阳性,并被鉴定为产毒氧化钾菌株。
■在伊朗被诊断患有癌症的儿童的粪便样本中产生毒素的K.oxytoca菌株的患病率相对较低。大多数氧化钾分离株对测试的抗生素敏感。全球范围内,建议在医疗机构中对不同恶性肿瘤患者或免疫功能低下患者进行产毒K.oxytoca菌株的主动监测.
UNASSIGNED: Klebsiella oxytoca (K. oxytoca) is the second bacterial cause of nosocomial infections in the general population after K. pneumoniae. This study surveyed the frequency of cytotoxin-producing strains of K. oxytoca and their antibiotic susceptibility profile in a cohort of children admitted to a referral hospital with different malignancies.
UNASSIGNED: The Stool samples of children admitted to the Cancer Chemotherapy Unit of the Mofid Children\'s Hospital, Tehran, Iran were analyzed using conventional biochemical tests and polymerase chain reaction targeting the pehX gene to identify K. oxytoca. The antibiotic susceptibility profile of isolated K. oxytoca against commonly prescribed antibiotics used in treating infection at the facility was determined using the Kirby-Bauer disk diffusion technique. Also, the prevalence of genes encoding toxins among K. oxytoca was identified by PCR assay.
UNASSIGNED: The Stool samples of 280 participants were taken for the study of which 38 samples [(55.3% (21/38) 42 males and 44.7% (17/38) females)] tested positive for various Klebsiella spp. Out of this, K. oxytoca was identified in 2.5% (7/280) stools using cultures and conventional biochemical tests. Also, the stools of 2.9% (8/280) of the participants tested positive for K. oxytoca using PCR assay. Using PCR, (2/7) of the K. oxytoca isolates tested positive for the npsA and npsB genes and were identified as toxigenic K. oxytoca strains.
UNASSIGNED: The prevalence of toxin-producing K. oxytoca strains in stool samples of children diagnosed with cancer in Iran is relatively low. Most of the K. oxytoca isolates were susceptible to tested antibiotics. Globally, active surveillance of toxigenic K. oxytoca strains in patients with different malignancies or immunocompromised patients is recommended in healthcare settings.