生酮饮食(KD)是一种高脂肪饮食,低碳水化合物饮食导致血清酮体升高,称为酮症。这种代谢结果已被建议作为治疗神经系统疾病的方法,改善锻炼表现,并促进超重个体的减肥。然而,由于大多数研究主要使用男性群体,人们对食用KD期间潜在的性别差异知之甚少。此外,KD对衰老的影响相对未被探索。因此,这项研究的目的是探讨饲喂KD的小鼠的性别和年龄特异性差异。将12周或24周龄的雄性和雌性C57BL/6N小鼠随机分配到KD(90%脂肪,1%碳水化合物)或食物(13%脂肪,60%碳水化合物)组为6周。KD诱导体重增加,肥胖增加,诱导高脂血症,导致心脏和肝脏中的脂质积累,导致心脏糖原消耗,肝脏,和肌肉根据年龄和性别有不同程度的变化。虽然KD上的年轻和老年雄性小鼠容易发生葡萄糖不耐受,KD大大提高了年轻女性的旋转性能。总的来说,这项研究强调了在适应KD方面潜在的性别和年龄差异。
The ketogenic diet (KD) is a high-fat, low-carbohydrate diet that results in the elevation of serum ketone bodies, known as ketosis. This metabolic consequence has been suggested as a method for treating neurological conditions, improving exercise performance, and facilitating weight loss for overweight individuals. However, since most research primarily uses male populations, little is known about the potential sex differences during the consumption of the KD. In addition, the effects of the KD on aging are relatively unexplored. Therefore, the purpose of this study was to explore sex- and age-specific differences in mice fed the KD. Male and female C57BL/6N mice at either 12 wks or 24 wks of age were randomly assigned to a KD (90% fat, 1% carbohydrate) or chow (13% fat, 60% carbohydrate) group for 6 wks. KD induced weight gain, increased adiposity, induced hyperlipidemia, caused lipid accumulation in the heart and liver, and led to glycogen depletion in the heart, liver, and muscle with varying degrees of changes depending on age and sex. While younger and older male mice on the KD were prone to glucose intolerance, the KD acutely improved rotarod performance in younger females. Overall, this study highlights potential sex and aging differences in the adaptation to the KD.