背景:产生角蛋白的牙源性囊肿(KPOCs)是一组通常具有侵袭性的囊性病变,高复发率和多病灶。KPOC包括正畸牙源性囊肿(OOC)和牙源性牙源性囊肿,现在被认为是真正的肿瘤,称为角化囊性牙源性肿瘤(KCOT)。GLUT1是一种蛋白质转运蛋白,参与跨细胞膜的葡萄糖的主动吸收,并且在肿瘤中过度表达,与增殖率和正电子发射断层扫描(PET)成像结果密切相关。
方法:对一组58个产生角蛋白的牙源性囊肿进行了GLUT1表达的组织学和免疫组织化学评估。使用β回归模型将不同数据与GLUT1的组织学类型和免疫组织化学表达相关,使用两种不同的形态学方法对其进行定量。
结果:KPOC病例包括12个OOC和46个KCOT,后者对应于6例综合征和40例零星KCOT。与KCOT病例相比,OOC病例中GLUT1表达非常低,考虑定量时,具有统计学显著性差异。通过免疫染色揭示了不同的GLUT1定位模式,在KCOT中表现出更高的反应性。然而,在KCOT案件中,GLUT1的表达无法确定综合征和散发性病例之间的差异。
结论:GLUT1表达在OOC和KCOT病例之间有差异,在KCOT中表达明显更高,但没有区分综合征和散发性KCOT病例。然而,考虑到KCOT的结构特征,我们假设PET成像方法可能不是KCOT的有用诊断工具.KCOT系列中GLUT1表达和PET检查的进一步研究需要证实最后一个假设。
BACKGROUND: Keratin-producing odontogenic cysts (KPOCs) are a group of cystic lesions that are often aggressive, with high rates of recurrence and multifocality. KPOCs included orthokeratinised odontogenic cyst (OOC) and parakeratotic odontogenic cysts, which are now considered true tumours denominated keratocystic odontogenic tumours (KCOTs). GLUT1 is a protein transporter that is involved in the active uptake of glucose across cell membranes and that is overexpressed in tumours in close correlation with the proliferation rate and positron emission tomography (PET) imaging results.
METHODS: A series of 58 keratin-producing odontogenic cysts was evaluated histologically and immunohistochemically in terms of GLUT1 expression. Different data were correlated using the beta regression model in relation to histological type and immunohistochemical expression of GLUT1, which was quantified using two different morphological methods.
RESULTS: KPOC cases comprised 12 OOCs and 46 KCOTs, the latter corresponding to 6 syndromic and 40 sporadic KCOTs. GLUT1 expression was very low in OOC cases compared with KCOT cases, with statistical significant differences when quantification was considered. Different GLUT1 localisation patterns were revealed by immunostaining, with the parabasal cells showing higher reactivity in KCOTs. However, among KCOTs cases, GLUT1 expression was unable to establish differences between syndromic and sporadic cases.
CONCLUSIONS: GLUT1 expression differentiated between OOC and KCOT cases, with significantly higher expression in KCOTs, but did not differentiate between syndromic and sporadic KCOT cases. However, given the structural characteristics of KCOTs, we hypothesised that PET imaging methodology is probably not a useful diagnostic tool for KCOTs. Further studies of GLUT1 expression and PET examination in KCOT series are needed to confirm this last hypothesis.