黄牛羊逆转录病毒(JSRV),属于逆转录病毒科的betaretrovirus属,包括外源性和内源性jaagsiekte绵羊逆转录病毒(exJSRV和enJSRV,分别)。在前病毒基因组水平,exJSRV和enJSRV菌株具有高度的相似性,它们的主要变异区域是LTR,gag,和env基因。在这项研究中,第一次,我们调查并比较了这些enJSRV和exJSRV菌株之间CpG岛的分布。具体来说,我们分析了从GenBank®数据库获得的总共42个全长JSRV基因组序列,以使用MethPrimer软件鉴定exJSRV和enJSRV基因组中的CpG岛.我们的结果表明,两个JSRV菌株中的CpG岛主要分布在LTR中,gag,和env基因。在exJSRV中,66.66%(6/9),33.33%(3/9),和100%(9/9)的序列在LTR中呈现至少一个CpG岛,gag,env基因,分别,对于enJSRV来说,84.84%(28/33),57.57%(19/33),和96.96%(32/33)的序列在LTR中存在至少一个CpG岛,gag,和env基因。这些发现表明,分布,长度,exJSRV和enJSRV菌株的CpG岛遗传性状不同。在未来,有必要证明exJSRV和enJSRV基因组中这些基因中CpG岛的生物学意义。这将增强对CpG岛在表观遗传调控中的潜在作用的理解。
The jaagsiekte sheep retrovirus (JSRV), belonging to the betaretrovirus genus of the retroviridae family, includes both exogenous and endogenous jaagsiekte sheep retroviruses (exJSRV and enJSRV, respectively). At the proviral genome level, exJSRV and enJSRV strains have a high degree of similarity with their main variation regions being the LTR, gag, and env genes. In this study, for the first time, we investigated and compared the distribution of CpG islands between these enJSRV and exJSRV strains. Specifically, we analyzed a total of 42 full-length JSRV genomic sequences obtained from the GenBank® database to identify CpG islands in the exJSRV and enJSRV genomes using the MethPrimer software. Our results showed that the CpG islands in the two JSRV strains were mainly distributed in the LTR, gag, and env genes. In exJSRVs, 66.66% (6/9), 33.33% (3/9), and 100% (9/9) of the sequences presented at least one CpG island in LTR, gag, env genes, respectively, and for enJSRVs, 84.84% (28/33), 57.57% (19/33), and 96.96% (32/33) of the sequences presented at least one CpG island in the LTR, gag, and env genes. These findings suggested that the distribution, length, and genetic traits of CpG islands were different for the exJSRV and enJSRV strains. In future, it would be necessary to demonstrate the biological significance of CpG islands within these genes in exJSRV and enJSRV genomes. This will enhance understanding regarding the potential role of CpG islands in epigenetic regulation.