肠道感染是新生猪断奶过渡期间肠道疾病的主要原因。二氢杨梅素(DMY)是从藤茶中提取的一种天然黄酮醇化合物,具有许多生物活性,例如抗氧化和免疫调节功能。本研究的目的是研究饲粮中添加二氢杨梅素对生长性能的影响,豁免权,产肠毒素大肠杆菌(ETEC)攻击的断奶猪的肠道功能。总的来说,将24只断奶的DLY(Duroc×Landrace×Yorkshire)猪分配给3种处理。饲喂含300mg/kgDMY的基础日粮或基础日粮的猪口服灭菌培养物或ETEC(2.5×1011个菌落形成单位)。与ECON组的猪相比,日粮DMY补充显著提高了EDMY组的断奶猪的最终体重和平均日增重(ADG),但降低了腹泻发生率(p<0.05)。与ECON组相比,DMY还提高了干物质(DM)的消化率,乙醚提取物(EE),总能源(GE),EDMY组的灰分(p<0.05)。此外,DMY不仅显着降低了白蛋白/球蛋白的比例,而且还升高了免疫球蛋白的血清浓度(例如,与ECON组的猪相比,EDMY组的断奶猪中的IgA和IgG)(p<0.05)。有趣的是,绒毛高度,绒毛高度与隐窝深度之比(V:C),和粘膜碱性磷酸酶的活性,蔗糖酶,与ECON组比较,EDMY组的十二指肠和空肠的麦芽糖酶含量均较高(p<0.05)。重要的是,DMY显著升高空肠小带闭塞-1(ZO-1)的表达水平,claudin-1,阳离子氨基酸转运蛋白-1(CAT-1),与ECON组的猪相比,EDMY组的断奶猪和脂肪酸转运蛋白-1(FATP-1)(p<0.05)。此外,与ECON组相比,DMY增加了微生物SCFA代谢物的浓度(例如,乙酸和丙酸),但EDMY组盲肠中大肠杆菌的丰度降低(p<0.05)。膳食补充DMY可以减轻ETEC诱导的生长迟缓和肠道损伤,这归因于肠道营养消化和运输功能的改善以及微生物群的改善。
Enteric infection is a major cause of enteric disorder in neonatal pigs during the weaning transition. Dihydromyricetin (DMY) is a natural flavanonol compound extracted from Ampelopsis grossedentata with numerous biological activities such as antioxidative and immunomodulatory functions. The objective of this study was to investigate the effects of dietary dihydromyricetin supplementation on growth performance, immunity, and intestinal functions in weaned pigs challenged by enterotoxigenic Escherichia coli (ETEC). In total, 24 weaned DLY (Duroc × Landrace × Yorkshire) pigs were allotted to 3 treatments. Pigs fed with basal diet or basal diet containing 300 mg/kg DMY were orally infused with sterilized culture or ETEC (2.5 × 1011 colony-forming units). Dietary DMY supplementation significantly elevated the final weight and average daily gain (ADG) but reduced diarrhea incidence in the weaned pigs of the EDMY group compared to the pigs of the ECON group (p < 0.05). Compared to the ECON group, DMY also improved the digestibility of dry matter (DM), ether extract (EE), gross energy (GE), and ash of the EDMY group (p < 0.05). Moreover, DMY not only significantly decreased the ratio of albumin/globulin but also elevated serum concentrations of immunoglobulins (e.g., IgA and IgG) in the weaned pigs of the EDMY group compared to the pigs of the ECON group (p < 0.05). Interestingly, the villus height, the ratio of villus height to crypt depth (V:C), and the activities of mucosal alkaline phosphatase, sucrase, and maltase in the duodenum and jejunum of the EDMY group were higher than those in the ECON group (p < 0.05). Importantly, DMY significantly elevated the expression levels of jejunal zonula occludens-1 (ZO-1), claudin-1, cationic amino acid transporter-1 (CAT-1), and fatty acid transport protein-1 (FATP-1) in the weaned pigs of the EDMY group compared to the pigs of the ECON group (p < 0.05). Additionally, compared to the ECON group, DMY increased the concentrations of microbial SCFA metabolites (e.g., acetic acid and propanoic acid), but reduced the abundance of Escherichia coli in the cecum of the EDMY group (p < 0.05). Dietary DMY supplementation can attenuate the ETEC-induced growth retardation and intestinal injury, which was attributed to the amelioration of intestinal nutrient digestion and transport functions as well as the improved microbiota.