蚊子埃及伊蚊是引起巴基斯坦登革热等地方病的病毒的主要传播者。它也是黄热病等其他媒介传播疾病的原因,寨卡热病,还有基孔肯雅,这对全球人类健康产生了重大影响。在没有有效疫苗(黄热病除外)或药物的情况下,矢量控制方法,如昆虫不育技术(SIT),已被提议作为管理这些疾病的额外工具。蚊子SIT计划基于不育雄性的释放,重要的是雌性释放理想地为零或保持在最低限度,因为女性是咬人的,采血和传播病原体。最近,Ae.埃及伊蚊遗传性别鉴定菌株(GSS),有和没有重组抑制反转(Inv35),使用眼睛的颜色作为可选择的标记,男性有黑眼睛,女性有红眼睛。在本研究中,我们介绍了Ae的性别特征和Inv35。埃及伊蚊红眼GSS进入巴基斯坦基因组背景,旨在将其未来用于该国的SIT应用。两种渗入的菌株,红眼GSS-PAK和红眼GSS/Inv35-PAK,通过评估重组率等参数来评估其遗传稳定性和生物学质量,繁殖力,生育力,p和成人恢复,发展的时间,蛹的重量,生存,与巴基斯坦野生种群(PAK)相比,飞行能力。结果表明,Inv35的性别特征和重组抑制特性在渗入局部基因组背景后不受影响;然而,两个新构建的菌株的一些生物学特性受到影响,积极或消极,建议在将GSS渗入新的基因组背景后,在将其用于SIT田间试验或应用之前,应进行彻底的质量控制分析。还讨论了在针对埃及伊蚊的SIT应用中使用具有局部基因组背景的GSS的重要性。
The mosquito species Aedes aegypti is the primary transmitter of viruses that cause endemic diseases like dengue in Pakistan. It is also a cause of other vector-borne diseases like yellow fever, Zika fever, and chikungunya, which significantly impact human health worldwide. In the absence of efficient vaccines (except for yellow fever) or drugs, vector control methods, such as the sterile insect technique (SIT), have been proposed as additional tools for the management of these diseases. Mosquito SIT programs are based on the release of sterile males and it is important female releases to be ideally zero or to be kept at a minimum, since females are the ones that bite, blood-feed and transmit pathogens. Recently, an Ae. aegypti genetic sexing strain (GSS), with and without a recombination-suppressing inversion (Inv35), was developed using the eye color as a selectable marker, with males having black eyes and females red eyes. In the present study, we introgressed the sexing features and the Inv35 of the Ae. aegypti red-eye GSS into the Pakistani genomic background aiming to their future use for SIT applications in the country. Both introgressed strains, the Red-eye GSS-PAK and the Red-eye GSS/Inv35-PAK, were evaluated in respect to their genetic stability and biological quality by assessing parameters like recombination rate, fecundity, fertility, pupal and adult recovery, time of development, pupal weight, survival, and flight ability in comparison with a wild Pakistani population (PAK). The results suggest that the sexing features and the recombination suppression properties of Inv35 were not affected after their introgression into the local genomic background; however, some biological traits of the two newly constructed strains were affected, positively or negatively, suggesting that a thorough quality control analysis should be performed after the introgression of a GSS into a new genomic background prior to its use in SIT field trials or applications. The importance of using GSS with local genomic background for SIT applications against Aedes aegypti is also discussed.