研究针对腹部脂解的低水平激光治疗(LLLT)的效果。雌性Wistar大鼠每周两次(5周)直接在腹部皮肤中施用LLLT。除对照组(n=5)外,动物接受红色波长660nm的处理为(I)R3.3组(n=5):3.3J/cm2,和(II)R5组(n=5):5J/cm2,或红外波长808nm的处理为(III)IR3.3组(n=5):3.3J/cm2,和(IV)IR5组(n=5):5J/cm2。对腹部皮下和肝脏组织进行组织学评估。在肝组织中分析了硫代巴比妥酸反应性物质(TBARS)和过氧化氢酶(CAT)活性的水平。在外周血天冬氨酸转氨酶(AST),丙氨酸氨基转移酶(ALT),碱性磷酸酶(ALP),高密度脂蛋白(HDL),低密度脂蛋白(LDL),甘油三酯,和总胆固醇进行了调查。在骨髓中进行微核测定。除了IR3.3组,所有治疗组均降低了体重(p<0.001)。R5组降低了腹部皮下组织重量和厚度(p<0.05),尽管所有治疗组均减少了脂肪细胞的数量及其大小(p<0.001)。肝脏无组织学改变。甘油三酯和LDL水平没有变化。IR5组升高总胆固醇水平,降低HDL,ALT(均p<0.05),和AST水平(p<0.001)。IR3.3组ALP水平较高(p<0.01)。R3.3组增加了TBARS和CAT活性(p<0.05)。没有发现诱变效应。5J/cm2的红色激光治疗导致脂解,并且没有改变肝脏参数。
Investigate the effects of low-level lasers therapy (LLLT) aiming abdominal lipolysis. Female Wistar rats received applications of LLLT directly in the abdominal skin twice a week (5 weeks). Except the control group (n = 5), animals received treatments with red wavelength 660 nm being (I) R3.3 group (n = 5): 3.3 J/cm2, and (II) R5 group (n = 5): 5 J/cm2, or infrared wavelength 808 nm being (III) IR3.3 group (n = 5): 3.3 J/cm2, and (IV) IR5 group (n = 5): 5 J/cm2. Abdominal subcutaneous and liver tissues were evaluated histologically. Levels of thiobarbituric acid reactive substances (TBARS) and catalase (CAT) activity were analyzed in liver tissue. In the peripheral blood aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), high-density lipoprotein (HDL), low-density lipoprotein (LDL), triglycerides, and total cholesterol were investigated. Micronucleus assay was performed in the bone marrow. Except for the IR3.3 group, all treated groups reduced the body weight (p < 0.001). The R5 group reduced the abdominal subcutaneous tissue weight and thickness (p < 0.05), even though all treated groups reduced the number of adipocytes and its size (p < 0.001). No histological changes in the liver. There were no alterations in the triglycerides and LDL levels. The IR5 group increased the total cholesterol levels and decreased the HDL, ALT (both p < 0.05), and AST levels (p < 0.001). The group IR3.3 showed higher levels of ALP (p < 0.01). The R3.3 group increased the TBARS and CAT activity (p < 0.05). No mutagenic effects were found. The red laser treatment at 5 J/cm2 led to lipolysis and did not alter the liver\'s parameters.