未经证实:人类中性粒细胞同种抗原(HNA)的抗体参与了几种临床疾病的病理生理学,包括输血相关的急性肺损伤(TRALI)。同种免疫和自身免疫性中性粒细胞减少症,和发热的非溶血性输血反应导致中性粒细胞减少。同源抗原是在嗜中性粒细胞上的几种糖蛋白上表达的多态性结构,即,抗原HNA-1a,-1b,-1c,和Fc-γ-受体IIIb上的-1d;CD177上的HNA-2;胆碱转运蛋白样蛋白2上的HNA-3a和-3b;αMβ2整联蛋白的CD11b/αM亚基上的HNA-4a和-4b(CD11b/CD18,Mac-1,CR3);和CDL-5a和-5b(CD11a)中的αL-5b。白细胞功能相关分子(LFA)-1。目前,在印度,检测海航的诊断方法存在缺陷。这项研究旨在确定印度人的海航频率,估计同种免疫的风险,准备嗜中性粒细胞样本,可用于检测中性粒细胞减少症病例中的HNA抗体。
未经证实:从随机的1,054名献血者中采集EDTA血样。通过流式细胞术使用FITC标记的单克隆抗CD177在新鲜EDTA样品上对HNA-2进行表型分析。通过DNA测序和PCR-RFLP进行HNA-1(FCGR3B)基因分型。使用TaqαI和Bsp1286I限制酶通过PCR-RFLP对HNA-3(SLC44A2)和HNA-5(ITGAL)的抗原进行基因分型,分别,而HNA-4(ITGAM)通过PCR-SSP进行基因分型。
未授权:发现FCGR3B*01、FCGR3B*02和FCGR3B*03的等位基因频率分别为0.433、0.444和0.087。FCGR3B*01+*02+*03-是最常见的基因型(33.78%)。十个个体显示FCGR3B个体缺乏,而23人表现出过表达,即,FCGR3B*01+*02+*03+。FCGR3B*04和*05以0.002和0.024的频率发生。发现HNA-2是出现在98.8%人群中的高频抗原。4%的个体在其中性粒细胞上显示CD177的非典型表达。SLC44A2*01和SLC44A2*02的等位基因频率分别为0.812和0.188,ITGAM*01、ITGAM*02、ITGAL*01和ITGAL*02分别为0.9546、0.0454、0.2372和0.7628。
UNASSIGNED:这是印度首次报道献血者中HNA频率的研究。在本研究中鉴定出的中性粒细胞分型板将使我们能够在将来调查疑似的免疫性中性粒细胞减少症病例。
UNASSIGNED: Antibodies to human neutrophil alloantigens (HNA) are involved in the pathophysiology of several clinical conditions including transfusion-related acute lung injury (TRALI), alloimmune and autoimmune neutropenia, and febrile nonhemolytic transfusion reactions leading to neutropenia. The cognate antigens are polymorphic structures expressed on several glycoproteins on the neutrophils, i.e., antigens HNA-1a, -1b, -1c, and -1d on Fc-γ-receptor IIIb; HNA-2 on CD177; HNA-3a and -3b on choline transporter-like protein 2; HNA-4a and -4b on CD11b/αM subunit of the αMβ2-integrin (CD11b/CD18, Mac-1, CR3); and HNA-5a and -5b on αL-subunit (CD11a) of the αLβ2 integrin (CD11a/CD18), leukocyte function associated molecule (LFA)-1. Currently, there is a lacuna of diagnostic methods for detection of HNA in India. This study aimed to determine the HNA frequencies in
Indians, estimate the risk of alloimmunization, and prepare typed neutrophil panels, which can be used to detect HNA antibodies in neutropenia cases.
UNASSIGNED: EDTA blood samples were collected from random 1,054 blood donors. HNA-2 was phenotyped on fresh EDTA samples using FITC labelled monoclonal anti-CD177 by flowcytometry. HNA-1 (FCGR3B) genotyping was carried out by DNA sequencing and PCR-RFLP. Antigens of HNA-3 (SLC44A2) and HNA-5 (ITGAL) were genotyped by PCR-RFLP using TaqαI and Bsp1286I restriction enzymes, respectively, while HNA-4 (ITGAM) was genotyped by PCR-SSP.
UNASSIGNED: Allele frequencies of FCGR3B*01, FCGR3B*02, and FCGR3B*03 were found to be 0.433, 0.444, and 0.087, respectively. FCGR3B*01+*02+*03- was the most common genotype (33.78%). Ten individuals showed deficiency of FCGR3B individuals, while 23 showed hyperexpression, i.e., FCGR3B*01+*02+*03+. FCGR3B*04and *05 occurred with a frequency of 0.002 and 0.024. HNA-2 was found to be a high frequency antigen occurring in 98.8% population. Four percent individuals showed atypical expression of CD177 on their neutrophils. Allele frequencies of SLC44A2*01 and SLC44A2*02were 0.812 and 0.188, respectively, and that of ITGAM*01, ITGAM*02, ITGAL*01, and ITGAL*02 were 0.9546, 0.0454, 0.2372, and 0.7628, respectively.
UNASSIGNED: This is the first study in India to report the frequencies of HNA among blood donors. Typed neutrophil panels identified in the present study will enable us to investigate suspected cases of immune neutropenia in future.