腺病毒是一组多样化的病毒,可以在家禽中引起多种疾病,包括呼吸道和胃肠道感染。在火鸡(Meleagrisgallopavo)中,腺病毒通常会引起出血性肠炎,很少,包涵体肝炎。在这项研究中,我们调查了在埃及火鸡中传播的家禽腺病毒(FAdV)。在对500只鸟进行临床检查后,六邻体基因的一部分是从患病鸟类的50个样本中的4个(8%)中扩增出来的,并且选择产生强条带的一个扩增子用于测序。分子和系统发育分析显示该样品中的病毒属于血清型FAdV-8b。对制备的组织切片进行组织病理学和免疫组织化学检查以确认病理结果。患病的鸟表现出褶皱的羽毛,体重低,蹲下的姿势,和腹泻。大体检查发现脾脏有瘀点出血,肿胀苍白的肝脏,还有充血的肠道.显微镜检查显示存在嗜酸性和嗜碱性的核内包涵体,核固缩症,和肝脏中的凋亡体,拥塞,出血,和肺纤维化,和肠上皮细胞的脱落。肝脏中存在病毒抗原,肺,和肠通过免疫组织化学证实。据我们所知,这是关于埃及FAdV-8b引起的火鸡(混合转炉品种)包涵体肝炎爆发特征的第一份报告。这一发现引发了流行病学警报,需要进一步的研究,包括全基因组测序,追踪病毒的起源和遗传多样性。
Adenoviruses are a diverse group of viruses that can cause a variety of diseases in poultry, including respiratory and gastrointestinal infections. In turkeys (Meleagris gallopavo), adenoviruses commonly cause hemorrhagic enteritis and, rarely, inclusion body hepatitis. In this study, we investigated fowl adenoviruses (FAdVs) circulating in turkeys in Egypt. Following clinical examination of 500 birds, a portion of the hexon gene was amplified from four out of 50 samples from diseased birds (8%), and one amplicon that produced a strong band was selected for sequencing. Molecular and phylogenetic analysis revealed that the virus in that sample belonged to serotype FAdV-8b. Histopathological and immunohistochemical examinations of prepared tissue sections were performed to confirm the pathological findings. Diseased birds exhibited ruffled feathers, low body weight, a crouching posture, and diarrhea. Gross examination revealed petechial hemorrhage on the spleen, swollen pale liver, and congested intestine. Microscopic examination revealed the presence of eosinophilic and basophilic intranuclear inclusion bodies, nuclear pyknosis, and apoptotic bodies in the liver, congestion, hemorrhage, and fibrosis in the lungs, and desquamation of enterocytes. The presence of viral antigens in the liver, lungs, and intestine was confirmed by immunohistochemistry. To our knowledge, this is the first report of the characterization of an outbreak of inclusion body hepatitis in turkeys (hybrid converter breeds) due to FAdV-8b in Egypt. This finding raises an epidemiological alarm, necessitating further studies, including full-genome sequencing, to trace the virus\'s origin and genetic diversity.