■本研究的目的是研究席夫碱和普瑞巴林衍生物及其金属(Zn和Cu)配合物对癫痫严重程度的预处理,延迟时间,抽搐的持续时间,小鼠癫痫发作评分和存活率。
■为了实现目标,在特定的分子靶标上进行了类似物的分子对接研究,例如α-2δ受体(PDBID:6ND9);其揭示了类似物对其各自靶标的显著结合亲和力。根据对接信息,合成了所有普瑞巴林衍生物,并通过应用PTZ模型证实了体内抗癫痫作用,该模型优先考虑了负责生物活性的最关键要点.
测试化合物显著增加了第一次癫痫发作的潜伏期,并降低了整个身体的癫痫发作频率以及频繁的旋转和跳跃。此外,在接受PTZ的小鼠中使用普瑞巴林衍生物的治疗显着降低了癫痫发作的持续时间和癫痫发作评分。然而,它提高了小鼠的存活率。
■由于新合成的化合物在某些方面比母体药物更具活性;因此,该项目的扩展可以计划在未来探索药物的临床方面。
席夫碱和普瑞巴林衍生物及其Zn和Cu金属络合物的对接研究席夫碱和普瑞巴林衍生物及其金属(Zn和Cu)PTZ癫痫模型的预处理不同参数的观察,包括;癫痫的严重程度,延迟时间,抽搐的持续时间,小鼠的癫痫发作评分和存活率与母体药物相比,新合成的化合物更具活性。
UNASSIGNED: The goal of the present study is to examine pretreatment with Schiff bases and derivatives of pregabalin along with their metal (Zn and Cu) complexes on the severity of epilepsy, latency time, duration of convulsions, seizure score and survival rate in mice.
UNASSIGNED: To achieve the goal, a molecular docking study of analogues was carried out on a specific molecular target, such as the alpha-2δ receptor (PDB ID: 6ND9); which revealed the significant binding affinity of the analogs to their respective target. Based on the docking information, all pregabalin derivatives were synthesized and in-vivo antiepileptic effect was confirmed by applying the PTZ model that prioritized the most crucial significant points responsible for biological activity.
UNASSIGNED: The test compounds markedly increased the latency of the first seizure and reduced the frequency of seizures throughout the body and frequent spinning and jumps. Additionally, treatment with pregabalin derivatives in mice that received PTZ significantly reduced the duration of seizures and seizure score. However, it increased the survival rate of the mice.
UNASSIGNED: Since the newly synthesized compounds were more active as compared to the parent drug in some respects; therefore, the expansion of the project can be planned to explore clinical side of the drugs in the future.
Docking studies of Schiff bases and derivatives of Pregabalin along with their Zn and Cu metal complexesPretreatment with Schiff bases and derivatives of pregabalin along with their metal (Zn and Cu)PTZ Model of epilepsyObservation of different parameters including; the severity of epilepsy, latency time, duration of convulsions, seizure score and survival rate in miceNewly synthesized compounds were more active as compared to the parent drug.