■Ghrelin是一种被翻译后修饰的食欲肽。ghrelin的天然自身抗体(ghrelin-aAb)已在健康受试者中描述,在饮食失调和风湿性疾病中,具有潜在的临床相关性。尽管有这些重要的报道,关于患病率和生理作用的数据库很小,评估ghrelin-aAb的技术方法很少,鼓励各自的研究,以提高对潜在内分泌意义的认识。
■基于人生长素释放肽与报告基因的融合蛋白产生了一种新的免疫沉淀测定。用商业抗体验证测定质量。测定特征和基质效应进行了测定,包括天然ghrelin-aAb对冷冻的稳定性,稀释实验中的信号线性,以及不同矩阵的比较。三组血清样本进行ghrelin-aAb分析,包含来自健康受试者和1型或2型糖尿病患者的商业血清。
■新产生的ghrelin-aAb分析被证明是灵敏的,强大的和可靠的在广泛的浓度范围。血清和血浆的结果略有不同。来自血清的信号对冻融保持稳定,在稀释实验中。对异常值应用数学准则(P75+1.5倍IQR),在不同的人类队列中,阳性样本的平均患病率为11%-12%,没有明显的性别或疾病相关差异。
■一种新的诊断自身抗体检测方法在糖尿病患者和对照组中检测到ghrelin-aAb的患病率相似,表明对ghrelin的自身免疫在糖尿病中几乎没有作用,但可能与ghrelin信号传导至关重要的其他疾病有关。
UNASSIGNED: Ghrelin is an orexigenic peptide that becomes post-translationally modified. Natural autoantibodies to ghrelin (ghrelin-aAb) have been described in healthy subjects, in eating disorders and rheumatic diseases, with potential clinical relevance. Despite these important reports, the data base on the prevalence and physiological role is small and technical approaches for assessing ghrelin-aAb are few, encouraging respective research for improving knowledge on the potential endocrine significance.
UNASSIGNED: A novel immunoprecipitation assay was generated based on a fusion protein of human ghrelin with a reporter gene. Assay quality was verified with commercial antibodies. Assay characteristics and matrix effects were determined, including stability of natural ghrelin-aAb to freezing, signal linearity in dilution experiments, and comparison of different matrices. Three groups of serum samples were analyzed for ghrelin-aAb, comprising commercial sera from healthy subjects and patients with type 1 or type 2 diabetes mellitus.
UNASSIGNED: The newly generated ghrelin-aAb assay proved sensitive, robust and reliable over a broad concentration range. Results from serum and plasma differed slightly. The signals from serum remained stable towards freezing and thawing, and in dilution experiments. Applying a mathematical criterion for outliers (P75 + 1.5-times IQR), an average prevalence of 11%-12% of positive samples was identified in the different human cohorts, with no significant sex-or disease-related difference.
UNASSIGNED: A novel diagnostic autoantibody assay detected ghrelin-aAb with a similar prevalence in diabetic patients and controls, suggesting that autoimmunity to ghrelin plays little role in diabetes mellitus, but may be of relevance in other diseases where ghrelin signaling is essential.