草药自古以来就被用作药物,人们发现人体对草药的反应很好。在四氧嘧啶诱导的糖尿病大鼠范例的当前研究中,对丁苷的作用进行了研究。将总共30只Wistar大鼠随机分为以下组(n=6):I-正常;II-四氧嘧啶诱导的(50mg/kg);III-四氧嘧啶+丁丁25mg/kg;IV-四氧嘧啶+丁丁50mg/kg;V-丁丁本身50mg/kg。各种糖尿病参数(血糖,胰岛素,HbA1c),血脂谱,炎性(TNF-α,IL-1β,IL-6和NF-κB),抗氧化酶(CAT,SOD和GSH),氧化应激指标(MDA),凋亡标志物(caspase-3),肝标志物(ALT和AST),并评估了组织病理学变化。此外,进行分子对接和动力学以评估丁香与靶蛋白的相互作用。Butin治疗,在两种剂量下,显著恢复糖尿病大鼠的生化指标和胰腺组织病理学。它有效地调节血液参数,脂质分布,炎症标志物,凋亡,抗氧化酶活性,氧化应激,和肝脏标志物。分子对接显示,布丁与caspase-3(1NME)等蛋白质结合,NF-κB(1SVC),和血清胰岛素(4IBM)的结合亲和力为-7.4,-6.5和-8.2kcal/mol,分别。分子动力学模拟进一步表明,布丁诱导这些蛋白质的显着构象变化。Butin通过恢复生化平衡对四氧嘧啶诱导的糖尿病大鼠表现出潜在的作用,减少炎症,保护胰腺组织.其与参与细胞凋亡和炎症的关键蛋白的结合突出了其在糖尿病管理中的治疗潜力。
Herbs have been used as medicines since antiquity, and it has been discovered that the human body responds well to herbal remedies. Research on the effect of butin was conducted in the current study in the alloxan-induced diabetic rat paradigm. A total of 30 Wistar rats were randomly assigned into the following groups (n = 6): I-Normal; II-Alloxan-induced (50 mg/kg); III-Alloxan + butin 25 mg/kg; IV-Alloxan + butin 50 mg/kg; V-Butin per se 50 mg/kg. Various diabetic parameters (blood glucose, insulin, HbA1c), lipid profile, inflammatory (TNF-α, IL-1β, IL-6 and NF-κB), antioxidant enzymes (CAT, SOD and GSH), oxidative stress indicators (MDA), apoptosis marker (caspase-3), hepatic markers (ALT and AST), and histopathological changes were assessed. Additionally, molecular docking and dynamics were performed to evaluate the interaction of butin with target proteins. Butin treatment, at both doses, significantly restored biochemical parameters and preserved pancreatic histopathology in diabetic rats. It effectively modulated blood parameters, lipid profiles, inflammatory markers, apoptosis, antioxidant enzyme activity, oxidative stress, and hepatic markers. Molecular docking revealed that butin binds to proteins such as caspase-3 (1NME), NF-κB (1SVC), and serum insulin (4IBM) with binding affinities of - 7.4, - 6.5, and - 8.2 kcal/mol, respectively. Molecular dynamics simulations further suggested that butin induces significant conformational changes in these proteins. Butin exhibits potential effects against alloxan-induced diabetic rats by restoring biochemical balance, reducing inflammation, and protecting pancreatic tissue. Its binding to key proteins involved in apoptosis and inflammation highlights its therapeutic potential in diabetes management.