背景:该研究旨在通过涉及计算机的综合评估来评估荨麻精油(UDEO)的抗氧化和伤口愈合特性,在体外,和体内分析。还研究了UDEO的植物化学以鉴定至关重要的痕量化合物。
方法:研究了色谱中多模式入口(MMI)的各种进样方法,以达到较低的仪器检测限。随后,采用计算机模拟研究来深入研究已鉴定化合物的潜在生物活性。标准抗氧化试验,包括ABTS·+和TAC,被执行了。使用大鼠模型实施以伤口愈合为中心的体内测试。将大鼠随机分为四组:生理盐水,凡士林车,胞嘧啶,和5%UDEO软膏。通过色度研究评估伤口愈合进展。
结果:气相色谱结合三重四极杆质谱(GC-MS/MS)分析显示UDEO中存在97种不耐热化合物。随后的计算机模拟研究揭示了鉴定的化合物抑制COX-2,TNF-α,和IL-6,表明抗炎反应和愈合过程的可能增强。体外测试阐明了UDEO的显着抗氧化能力,伤口愈合数据强化了这一发现,在局部应用UDEO后,显示出89%的实质性闭合率。值得注意的是,纤维蛋白原和C反应蛋白(CRP)水平显着降低,表明与对照相比,氧化应激损伤最小化。此外,与对照相比,UDEO表现出抗氧化酶活性的增加。
结论:该研究得出结论,UDEO具有显着的抗氧化和伤口愈合特性,由其丰富的植物化学成分支持。这些发现表明其在氧化应激和炎症条件的治疗干预中的潜在应用。
BACKGROUND: The study aimed to assess the antioxidant and wound healing properties of Urtica dioica essential oil (UDEO) through a comprehensive evaluation involving in silico, in vitro, and in vivo analyses. The phytochemistry of UDEO was also investigated to identify trace compounds crucial.
METHODS: Various injection methods of the multimode inlet (MMI) in chromatography were investigated to attain lower instrumental detection limits. Subsequently, in silico studies were employed to delve deeper into the potential biological activities of the identified compounds. Standard antioxidative tests, encompassing ABTS•+ and TAC, were performed. In vivo tests centered on wound healing were implemented using rat models. The rats were randomly allocated to four groups: saline solution, vaseline vehicle, cytol centella, and 5% UDEO ointment. Wound healing progress was evaluated through a chromatic study.
RESULTS: Gas chromatography combined with triple quadrupole mass spectrometry (GC-MS/MS) analysis revealed the presence of 97 thermolabile compounds in UDEO. Subsequent in silico studies unveiled the potential of identified compounds to inhibit COX-2, TNF-α, and IL-6, suggesting a possible enhancement of anti-inflammatory responses and healing processes. In vitro tests elucidated the notable antioxidant capacity of UDEO, a finding reinforced by wound healing data, revealing a substantial closure rate of 89% following the topical application of UDEO. Notably, fibrinogen and C-reactive protein (CRP) levels were significantly reduced, indicating minimized oxidative stress damage compared to control. Additionally, UDEO exhibited an increase in antioxidant enzyme activities compared to control.
CONCLUSIONS: The study concludes that UDEO possesses significant antioxidant and wound-healing properties, supported by its rich phytochemical composition. The findings suggest its potential application in therapeutic interventions for oxidative stress and inflammatory conditions.